Natural variation in the promoter of GsERD15B affects salt tolerance in soybean.

Natural variation in the promoter of GsERD15B affects salt tolerance in soybean.
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DOI:
10.1111/pbi.13536
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发表时间:
2021-06
影响因子:
13.8
通讯作者:
Li Y
Li Y
中科院分区:
工程技术1区
文献类型:
--
作者:
Jin T;Sun Y;Shan Z;He J;Wang N;Gai J;Li Y

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盐胁迫对作物生长和产量产生不利影响,受盐影响的土地面积不断增加。大豆是植物蛋白、油脂和饲料的主要来源,但被认为是盐敏感作物。栽培大豆(Glycine max)是由野生大豆(G. soja)驯化而来,但在人工选择过程中失去了大量的遗传多样性。因此,开发野生大豆基因库具有重要意义。在这项研究中,我们从野生大豆种质中鉴定了34个耐盐品种,发现GsERD15B启动子中一个7 bp的插入/缺失(InDel)(对脱水15B的早期反应)显著影响大豆的耐盐性。GsERD15B编码一种具有转录激活功能的蛋白,并包含一个PAM2结构域,介导其与聚(a)结合(PAB)蛋白的相互作用。GsERD15B启动子7 bp的缺失增强了大豆的耐盐性,使GsERD15B、两个GmPAB基因以及已知的胁迫相关基因GmABI1、GmABI2、GmbZIP1、GmP5CS、GmCAT4、GmPIP1:6、GmMYB84和GmSOS1在盐胁迫下的表达上调。我们认为,GsERD15B启动子的自然变异影响大豆的耐盐性,而过表达GsERD15B可能通过增加ABA信号、脯氨酸含量、过氧化氢酶过氧化物酶、脱水反应和阳离子运输相关基因的表达水平来增强大豆的耐盐性。
Salt stress has detrimental effects on crop growth and yield, and the area of salt‐affected land is increasing. Soybean is a major source of vegetable protein, oil and feed, but considered as a salt‐sensitive crop. Cultivated soybean (Glycine max) is domesticated from wild soybean (G. soja) but lost considerable amount of genetic diversity during the artificial selection. Therefore, it is important to exploit the gene pool of wild soybean. In this study, we identified 34 salt‐tolerant accessions from wild soybean germplasm and found that a 7‐bp insertion/deletion (InDel) in the promoter of GsERD15B (early responsive to dehydration 15B) significantly affects the salt tolerance of soybean. GsERD15B encodes a protein with transcriptional activation function and contains a PAM2 domain to mediate its interaction with poly(A)‐binding (PAB) proteins. The 7‐bp deletion in GsERD15B promoter enhanced the salt tolerance of soybean, with increased up‐regulation of GsERD15B, two GmPAB genes, the known stress‐related genes including GmABI1, GmABI2, GmbZIP1, GmP5CS, GmCAT4, GmPIP1:6, GmMYB84 and GmSOS1 in response to salt stress. We propose that natural variation in GsERD15B promoter affects soybean salt tolerance, and overexpression of GsERD15B enhanced salt tolerance probably by increasing the expression levels of genes related to ABA‐signalling, proline content, catalase peroxidase, dehydration response and cation transport.
GsCHX19.3是来自野生大豆的阳离子/H交换超家族的成员,有助于高盐度和碳酸盐碱性耐受性
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DOI: 10.1111/pbi.13536
发表时间: 2021-06
影响因子: 13.8
作者:
Jin T;Sun Y;Shan Z;He J;Wang N;Gai J;Li Y
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