Physical Location of New PCR-Based Markers and Powdery Mildew Resistance Gene(s) on Rye (Secale cereale L.) Chromosome 4 Using 4R Dissection Lines.
Physical Location of New PCR-Based Markers and Powdery Mildew Resistance Gene(s) on Rye (Secale cereale L.) Chromosome 4 Using 4R Dissection Lines.
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DOI：
10.3389/fpls.2017.01716
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发表时间：
2017
影响因子：
5.6
通讯作者：
Tang ZX
中科院分区：
文献类型：
--
作者：
Duan Q;Wang YY;Qiu L;Ren TH;Li Z;Fu SL;Tang ZX
黑麦（Secale cereale L.）4 R染色体含有适用于小麦的优良基因。品种改良基于PCR的4 R特异性标记有助于小麦背景中4 R上优良基因的检测。在这项研究中，一个新的荧光原位杂交（FISH）图谱的4 RKu染色体的黑麦Kustro已建成。获得了一组4 RKu分离系，利用特异长度扩增片段测序（SLAF-seq）技术开发了301个新的4 RKu特异标记。将这些标记与先前开发的99个4 RKu特异性标记组合，并使用新的FISH图谱和4 RKu解剖系物理定位到4 RKu染色体。400个标记中的338个已成功定位到4 RKu染色体的6个区域。此外，4 RLKu臂上的白粉病抗性基因定位于L.4和L.8之间的片段，与定位115个4 RLKu特异性标记的区域相同。本研究开发的标记可用于小麦背景中4 R染色质的特异片段的鉴定，有助于构建4 R染色体的高密度物理图谱，为4 R染色体上的优良基因在小麦育种中的利用提供了便利。
Rye (Secale cereale L.) 4R chromosome contains elite genes that are applicable for wheat (Triticum aestivum L.) cultivar improvement. PCR-based 4R-specific markers can benefit the detection of elite genes on 4R in wheat backgrounds. In this study, a new fluorescence in situ hybridization (FISH) map of the 4RKu chromosome of rye Kustro has been constructed. A set of 4RKu dissection lines was obtained and 301 new 4RKu-specific markers were developed using specific length amplified fragment sequencing (SLAF-seq) technology. These markers were combined with the 99 4RKu-specific markers previously developed, and were physically mapped to 4RKu chromosome using the new FISH map and the 4RKu dissection lines. A total of 338 of the 400 markers have been successfully mapped to six regions of 4RKu chromosome. Additionally, the powdery mildew resistance gene(s) on the 4RLKu arm was located to the segment between L.4 and L.8, the same region where 115 4RLKu-specific markers were mapped. The markers developed in this study can be used to identify a specific segment of 4R chromatin in wheat backgrounds, help construct a high-density physical map of 4R chromosome, and facilitate the utilization of elite genes on 4R chromosome in wheat breeding programs.
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影响因子：
2
作者：
Landjeva, S;Korzun, V;Ganeva, G
通讯作者：
Ganeva, G
抗白粉病小麦-黑麦4R染色体易位新系的分子细胞遗传学特征
影响因子：
2.6
作者：
An, Diaoguo;Zheng, Qi;Xu, Yunfeng
通讯作者：
Xu, Yunfeng
DOI：
10.1007/bf02172376
发表时间：
1996-06-24
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
DeScenzo, RA;Wise, RP
通讯作者：
Wise, RP
影响因子：
5.4
作者：
Ma, XF;Wanous, MK;Gustafson, JP
通讯作者：
Gustafson, JP
影响因子：
1.1
作者：
Ashida, Taizo;Nasuda, Shuhei;Endo, Takashi R.
通讯作者：
Endo, Takashi R.