Tonic Serotonin Measurements In Vivo Using N-Shaped Multiple Cyclic Square Wave Voltammetry.
Tonic Serotonin Measurements In Vivo Using N-Shaped Multiple Cyclic Square Wave Voltammetry.
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DOI：
10.1021/acs.analchem.1c02131
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发表时间：
2021-12-28
影响因子：
7.4
通讯作者：
Lee, Kendall H.
中科院分区：
文献类型：
--
作者：
Shin, Hojin;Goyal, Abhinav;Barnett, J. Hudson;Rusheen, Aaron E.;Yuen, Jason;Jha, Rohan;Hwang, Sang Mun;Kang, Yumin;Park, Cheonho;Cho, Hyun-U;Blaha, Charles D.;Bennet, Kevin E.;Oh, Yoonbae;Heien, Michael L.;Jang, Dong Pyo;Lee, Kendall H.
在这里，我们介绍了一种新的伏安技术-N形多循环方波伏安法(N-MCSWV)的发展及其在体内的应用。它可以定量测量体内紧张性细胞外5-羟色胺水平，减轻污垢影响。N-MCSWV通过产生高维伏安图丰富了电化学信息，使得对5-羟基吲哚乙酸(5-HIAA)、多巴胺、3，4-二羟基苯乙酸(DOPAC)、组胺、抗坏血酸、去甲肾上腺素、腺苷和pH具有高的灵敏度和选择性。用N-MCSWV结合PEDOT：Nafion包被的碳纤维微电极，测定了乌拉坦麻醉大鼠黑质网状部5-羟色胺浓度为52±5.8 nM(n=20)。多巴胺能、去甲肾上腺素和5-羟色胺能突触再摄取抑制剂的药理学挑战支持了N-MCSWV选择性检测体内紧张性5-羟色胺水平的能力。总体而言，N-MCSWV是一种用于5-羟色胺分析定量的新的伏安技术。它提供了对大脑中紧张性5-羟色胺浓度变化的持续监测，以进一步了解5-羟色胺在正常行为和精神障碍中的作用。
Here, we present the development of a novel voltammetric technique, N-shaped multiple cyclic square wave voltammetry (N-MCSWV) and its application in vivo. It allows quantitative measurements of tonic extracellular levels of serotonin in vivo with mitigated fouling effects. N-MCSWV enriches the electrochemical information by generating high dimensional voltammograms, which enables high sensitivity and selectivity against 5-hydroindoleacetic acid (5-HIAA), dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC), histamine, ascorbic acid, norepinephrine, adenosine, and pH. Using N-MCSWV, in combination with PEDOT:Nafion-coated carbon fiber microelectrodes, a tonic serotonin concentration of 52 ± 5.8 nM (n = 20 rats, ±SEM) was determined in the substantia nigra pars reticulata of urethane-anesthetized rats. Pharmacological challenges with dopaminergic, noradrenergic, and serotonergic synaptic reuptake inhibitors supported the ability of N-MCSWV to selectively detect tonic serotonin levels in vivo. Overall, N-MCSWV is a novel voltammetric technique for analytical quantification of serotonin. It offers continuous monitoring of changes in tonic serotonin concentrations in the brain to further our understanding of the role of serotonin in normal behaviors and psychiatric disorders.
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改善5-羟色胺快速扫描循环伏安法检测：新波形以减少电极结垢。
DOI：
10.1039/d0an01406k
发表时间：
2020-11-09
期刊：
The Analyst
影响因子：
--
作者：
Dunham KE ;Venton BJ
通讯作者：
Venton BJ
影响因子：
3.1
作者：
Kim, Do Hyoung;Oh, Yoonbae;Jang, Dong Pyo
通讯作者：
Jang, Dong Pyo
影响因子：
3
作者：
BLAHA, CD;PHILLIPS, AG
通讯作者：
PHILLIPS, AG
影响因子：
5
作者：
Burrell, Mark H.;Atcherley, Christopher W.;Lipski, Janusz
通讯作者：
Lipski, Janusz
影响因子：
3
作者：
JUSTICE, JB
通讯作者：
JUSTICE, JB