A therapeutic strategy for spinal cord defect: human dental follicle cells combined with aligned PCL/PLGA electrospun material.
A therapeutic strategy for spinal cord defect: human dental follicle cells combined with aligned PCL/PLGA electrospun material.
复制标题
脊髓缺陷的治疗策略：人牙囊细胞与对齐的 PCL/PLGA 电纺材料相结合。
DOI：
10.1155/2015/197183
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发表时间：
2015
影响因子：
--
通讯作者：
Tian W
中科院分区：
文献类型：
--
作者：
Li X;Yang C;Li L;Xiong J;Xie L;Yang B;Yu M;Feng L;Jiang Z;Guo W;Tian W
干细胞移植已被用于脊髓损伤的修复，但在修复器官的大规模损伤方面表现不佳。我们假设牙囊细胞具有多潜能，可与生物材料复合修复脊髓缺损区。本研究对人树突状细胞(HDFCs)的间充质和神经源性谱系特征进行了鉴定。制备了电纺取向的PCL/PLGA材料(AEM)，该材料不会引起细胞毒性反应，并且hDFCs可以沿取向纤维伸展并在AEM上高效增殖。随后，将种植于AEM的hDFCs移植到大鼠脊髓缺损处。进行功能观察，但结果无统计学意义。随后的组织学分析证明，AEM允许神经纤维通过，植入的hDFCs在体内可以表达能够促进重新髓鞘形成的少突胶质细胞系标志物Orig2。因此，hDFCs可以作为神经再生的候选来源。定向电纺纤维可支持脊髓结构，诱导细胞/组织极化。这一策略可作为SCD再生研究的替代方案。
Stem cell implantation has been utilized for the repair of spinal cord injury; however, it shows unsatisfactory performance in repairing large scale lesion of an organ. We hypothesized that dental follicle cells (DFCs), which possess multipotential capability, could reconstruct spinal cord defect (SCD) in combination with biomaterials. In the present study, mesenchymal and neurogenic lineage characteristics of human DFCs (hDFCs) were identified. Aligned electrospun PCL/PLGA material (AEM) was fabricated and it would not lead to cytotoxic reaction; furthermore, hDFCs could stretch along the oriented fibers and proliferate efficiently on AEM. Subsequently, hDFCs seeded AEM was transplanted to restore the defect in rat spinal cord. Functional observation was performed but results showed no statistical significance. The following histologic analyses proved that AEM allowed nerve fibers to pass through, and implanted hDFCs could express oligodendrogenic lineage maker Olig2 in vivo which was able to contribute to remyelination. Therefore, we concluded that hDFCs can be a candidate resource in neural regeneration. Aligned electrospun fibers can support spinal cord structure and induce cell/tissue polarity. This strategy can be considered as alternative proposals for the SCD regeneration studies.
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DOI：
10.1007/s10856-010-4048-y
发表时间：
2010-06-01
影响因子：
3.7
作者：
Nguyen Thi Hiep;Lee, Byong-Taek
通讯作者：
Lee, Byong-Taek
人牙囊细胞和人牙乳头细胞牙源分化的比较
影响因子：
3.7
作者：
Guo L;Li J;Qiao X;Yu M;Tang W;Wang H;Guo W;Tian W
通讯作者：
Tian W
影响因子：
14
作者：
Sun, HF;Mei, L;Wang, PY
通讯作者：
Wang, PY
影响因子：
64.5
作者：
Lu P;Wang Y;Graham L;McHale K;Gao M;Wu D;Brock J;Blesch A;Rosenzweig ES;Havton LA;Zheng B;Conner JM;Marsala M;Tuszynski MH
通讯作者：
Tuszynski MH
DOI：
10.1523/jneurosci.4967-10.2011
发表时间：
2011-03-16
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Takeoka A;Jindrich DL;Muñoz-Quiles C;Zhong H;van den Brand R;Pham DL;Ziegler MD;Ramón-Cueto A;Roy RR;Edgerton VR;Phelps PE
通讯作者：
Phelps PE