Evolution of lbx spinal cord expression and function.
Evolution of lbx spinal cord expression and function.
复制标题
脊髓lbx表达和功能的演变。
DOI：
10.1111/ede.12387
复制
发表时间：
2021-09
影响因子：
2.9
通讯作者：
Lewis KE
中科院分区：
文献类型：
--
作者：
Juárez-Morales JL;Weierud F;England SJ;Demby C;Santos N;Grieb G;Mazan S;Lewis KE
瓢虫同源盒（Lbx）转录因子在许多动物的肌肉和神经系统发育中具有重要的功能。羊膜有两个Lbx基因，但只有Lbx 1在脊髓中表达。与此相反，硬骨鱼有三个lbx基因，我们在这里显示，斑马鱼lbx 1a，lbx 1b和lbx 2由不同的脊髓细胞类型表达，lbx 1a在dI 4，dI 5和dI 6 interneurons中表达，如在斑马鱼。我们的数据研究lbx在Scyliorhinus canicula和热带爪蟾的表达表明，斑马鱼lbx 1a的脊髓中间神经元的表达是祖先的，而lbx 1b已获得一个新的表达模式在脊髓祖细胞。lbx 2脊髓表达可能是在鳍条鱼谱系中获得的，因为该基因在黄鳍鱼或黄鳍鱼的脊髓中不表达。小锥我们还表明，斑马鱼lbx 1a的脊髓功能与小鼠lbx 1是保守的。在斑马鱼Lbx 1a突变体中，抑制性脊髓中间神经元的数量减少，兴奋性脊髓中间神经元的数量增加，与小鼠Lbx 1突变体相似。有趣的是，在lbx 1b突变体中抑制性脊髓中间神经元的数量也减少了，尽管在这种情况下兴奋性中间神经元的数量没有增加。LBX 1A; LBX 1B双突变体具有与LBX 1A单突变体相似的脊髓中间神经元表型。总之，这些数据表明，lbx 1b和lbx 1a可能需要在连续的dI 4和dI 6脊髓中间神经元的正确规范，虽然只有lbx 1a是需要在这些细胞中的兴奋性命运的抑制。
Ladybird homeobox (Lbx) transcription factors have crucial functions in muscle and nervous system development in many animals. Amniotes have two Lbx genes, but only Lbx1 is expressed in spinal cord. In contrast, teleosts have three lbx genes and we show here that zebrafish lbx1a, lbx1b and lbx2 are expressed by distinct spinal cell types, and that lbx1a is expressed in dI4, dI5 and dI6 interneurons, as in amniotes. Our data examining lbx expression in Scyliorhinus canicula and Xenopus tropicalis suggest that the spinal interneuron expression of zebrafish lbx1a is ancestral, whereas lbx1b has acquired a new expression pattern in spinal cord progenitor cells. lbx2 spinal expression was probably acquired in the ray-finned lineage, as this gene is not expressed in the spinal cords of either amniotes or S. canicula. We also show that the spinal function of zebrafish lbx1a is conserved with mouse Lbx1. In zebrafish lbx1a mutants, there is a reduction in the number of inhibitory spinal interneurons and an increase in the number of excitatory spinal interneurons, similar to mouse Lbx1 mutants. Interestingly, the number of inhibitory spinal interneurons is also reduced in lbx1b mutants, although in this case the number of excitatory interneurons is not increased. lbx1a;lbx1b double mutants have a similar spinal interneuron phenotype to lbx1a single mutants. Taken together these data suggest that lbx1b and lbx1a may be required in succession for correct specification of dI4 and dI6 spinal interneurons, although only lbx1a is required for suppression of excitatory fates in these cells.
登录
查看更多内容
影响因子：
3
作者：
Juarez-Morales，Jose L.;Martinez-De Luna，Reyna I.;Lewis，Katharine E.
通讯作者：
Lewis，Katharine E.
DOI：
10.1101/pdb.emo111
发表时间：
2008-12-01
期刊：
CSH protocols
影响因子：
--
作者：
Coolen, Marion;Menuet, Arnaud;Mazan, Sylvie
通讯作者：
Mazan, Sylvie
新型小鸡同源基因 lbx3 的克隆和表达模式
影响因子：
1.2
作者：
Kanamoto, T;Terada, K;Furukawa, T
通讯作者：
Furukawa, T
影响因子：
3.6
作者：
Hilinski WC;Bostrom JR;England SJ;Juárez-Morales JL;de Jager S;Armant O;Legradi J;Strähle U;Link BA;Lewis KE
通讯作者：
Lewis KE
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF