Gallic acid-gold nanoparticles enhance radiation-induced cell death of human glioma U251 cells.
Gallic acid-gold nanoparticles enhance radiation-induced cell death of human glioma U251 cells.
复制标题
没食子酸-金纳米颗粒增强人胶质瘤U251细胞辐射诱导的细胞死亡。
作者：
Jing Z;Li M;Wang H;Yang Z;Zhou S;Ma J;Meng E;Zhang H;Liang W;Hu W;Wang X;Fu X
多形性胶质母细胞瘤(GBM)是最常见的成人脑肿瘤之一，具有致命性。然而，在有限的传统治疗之后，几乎所有的患者在复发时都有症状。开发新的治疗策略来改进目前的GBM治疗是非常紧迫的。没食子酸是一种公认的抗氧化剂，是一种很有前途的新型选择性抗癌药物，而纳米金(GNPs)可以作为一种多功能的无毒载体来传递抗癌药物。在这里，我们将没食子酸负载到四氯金酸被柠檬酸钠还原的GNPs上，通过物理吸附和搅拌吸附制备了没食子酸-GNPs(GA-GNPs)。GA-GNPs，而不是单独的GNPs，显著抑制U251 GBM细胞的存活，并增强辐射诱导的细胞死亡。此外，GA-GNPs联合放射治疗可使U251细胞周期停滞在S和G2/M期，并诱导细胞死亡，这种作用与bax蛋白水平升高和bcl2表达降低有关。因此，GA-GNPs与放射治疗联合应用于骨肉瘤的治疗具有很大的潜力。
Glioblastoma multiforme (GBM) is among the most common adult brain tumors with invariably fatal character. Following the limited conventional therapies, almost all patients, however, presented with symptoms at the time of recurrence. It is dire to develop novel therapeutic strategies to improve the current treatment of GBM. Gallic acid is a well‐established antioxidant, presenting a promising new selective anti‐cancer drug, while gold nanoparticles (GNPs) can be developed as versatile nontoxic carriers for anti‐cancer drug delivery. Here, we prepared gallic acid‐GNPs (GA‐GNPs) by loading gallic acid onto GNPs, reduction products of tetrachloroauric acid by sodium citrate, through physical and agitation adsorption. GA‐GNPs, rather than GNPs alone, significantly inhibited the survival of U251 GBM cells, as well as enhanced radiation‐induced cell death. Moreover, GA‐GNPs plus radiation arrested the cell cycle of U251 at the S and G2/M phases and triggered apoptotic cell death, which is supported by increased BAX protein levels and decreased expression of BCL‐2. Thus, GA‐GNPs have great potential in the combination with radiation therapy in future studies for GBM treatment.
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中国205例多形性胶质母细胞瘤患者的生存分析：临床特征、治疗和预后
影响因子：
2
作者：
Ma, Xiangyu;Lv, Yafeng;Li, Xingang
通讯作者：
Li, Xingang
影响因子：
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作者：
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通讯作者：
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影响因子：
3.9
作者：
Shukla, R;Bansal, V;Sastry, M
通讯作者：
Sastry, M
DOI：
10.1007/978-1-4939-6646-2_2
发表时间：
2017-01-01
期刊：
CANCER NANOTECHNOLOGY: METHODS AND PROTOCOLS
影响因子：
--
作者：
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通讯作者：
Kamaly, Nazila
影响因子：
5
作者：
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To, Shing-Shun Tony