Noninvasive imaging of infection after treatment with tumor-homing bacteria using Chemical Exchange Saturation Transfer (CEST) MRI.
Noninvasive imaging of infection after treatment with tumor-homing bacteria using Chemical Exchange Saturation Transfer (CEST) MRI.
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DOI：
10.1002/mrm.24955
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发表时间：
2013-12
影响因子：
3.3
通讯作者：
van Zijl, Peter C. M.
中科院分区：
文献类型：
--
作者：
Liu, Guanshu;Bettegowda, Chetan;Qiao, Yuan;Staedtke, Verena;Chan, Kannie W. Y.;Bai, Renyuan;Li, Yuguo;Riggins, Gregory J.;Kinzler, Kenneth W.;Bulte, Jeff W. M.;McMahon, Michael T.;Gilad, Assaf A.;Vogelstein, Bert;Zhou, Shibin;van Zijl, Peter C. M.
利用内源性CEST造影剂，开发一种非侵入性MRI方法，用于确定溶菌治疗中肿瘤归巢细菌的萌发和感染。首先在体外对厌氧革兰氏阳性细菌Clostridium novyi-NT （C. novyi-NT）的CEST参数进行表征，然后用3亿个细菌孢子检测CT26 （n=6）结直肠肿瘤小鼠皮下C. novyi-NT的萌发和感染情况。采用脂多糖（LPS）注射小鼠，排除CEST MRI变化是由炎症引起的。体外细菌悬浮液在较宽的频率范围内观察到CEST对比，与水共振的最大对比度约为2.6 ppm。细菌孢子未检测到信号，证明了萌发的特异性。在体内，与细菌萌发和感染前相比，C. novyi-NT感染肿瘤的CEST对比显著升高（p<0.05, n=6）。lps诱导的无菌性炎症无明显变化（p < 0.05, n=4）。内源性细菌CEST对比（bacCEST）可用于监测治疗性细菌C. novyi-NT的萌发和增殖，而不需要外源性细胞标记探针。
To develop a non-invasive MRI method for determining the germination and infection of tumor-homing bacteria in bacteriolytic cancer therapy using endogenous CEST contrast. The CEST parameters of the anaerobic gram-positive bacterium Clostridium novyi-NT (C. novyi-NT) were first characterized in vitro, then used to detect C. novyi-NT germination and infection in subcutaneous CT26 colorectal tumor-bearing mice (n=6) after injection of 300 million bacterial spores. Lipopolysacharide (LPS) injected mice were used to exclude that the changes of CEST MRI were due to inflammation. CEST contrast was observed over a broad frequency range for bacterial suspensions in vitro, with the maximum contrast around 2.6 ppm from the water resonance. No signal could be detected for bacterial spores, demonstrating the specificity for germination. In vivo, a significant elevation of CEST contrast was identified in C. novyi-NT infected tumors as compared to those before bacterial germination and infection (p<0.05, n=6). No significant change was observed in tumors with LPS-induced sterile inflammation (p> 0.05, n=4). Endogenous bacterial CEST contrast (bacCEST) can be used to monitor the germination and proliferation of the therapeutic bacterium C. novyi-NT without a need for exogenous cell labeling probes.
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影响因子：
3.6
作者：
Contag, CH;Contag, PR;Benaron, DA
通讯作者：
Benaron, DA
影响因子：
3.5
作者：
Dehoux, MJM;van Beneden, RP;Thissen, JPM
通讯作者：
Thissen, JPM
影响因子：
4.8
作者：
Dev, R;Gupta, RK;Husain, M
通讯作者：
Husain, M
影响因子：
19.7
作者：
Himmelreich, U;Accurso, R;Mountford, CE
通讯作者：
Mountford, CE
DOI：
10.1073/pnas.0406242101
发表时间：
2004-10-19
影响因子：
11.1
作者：
Agrawal, N;Bettegowda, C;Vogelstein, B
通讯作者：
Vogelstein, B