Mutagenesis of the orco odorant receptor co-receptor impairs olfactory function in the malaria vector Anopheles coluzzii.
Mutagenesis of the orco odorant receptor co-receptor impairs olfactory function in the malaria vector Anopheles coluzzii.
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DOI：
10.1016/j.ibmb.2020.103497
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发表时间：
2020-12
影响因子：
3.8
通讯作者：
Zwiebel LJ
中科院分区：
文献类型：
--
作者：
Sun H;Liu F;Ye Z;Baker A;Zwiebel LJ
蚊子在很大程度上依赖于它们的嗅觉系统来寻找宿主，选择产卵地点，避免捕食者和其他环境危险。在这些行为中，对人类血餐宿主的优先选择驱动了嗜中性雌性科氏按蚊的媒介能力。嗅觉受体神经元（ORN）分散在头部的几个附属物上，并表达与“调谐”气味受体（OR）偶联的专性气味受体共受体（Orco），以在这些神经元的膜中形成异聚体的气味门控离子通道。研究Orco/OR复合物对An的化学感受过程的机制和功能贡献。在coluzzii中，我们利用CRISPR/Cas9基因编辑来创建一系列纯合的Orco敲除突变蚊子。正如预期的那样，orco−/− ORN跨越了An的成虫和幼虫阶段。coluzzii显示出明显较低的背景活性，并且几乎没有所有的气味诱发反应。此外，吸血的成年雌性orco−/−突变蚊子对人类和非人类来源的气味的吸引力严重降低，而怀孕的雌性对已建立的产卵引诱剂的反应显着降低。这些结果加强了在其他昆虫中的观察，即Orco在维持ORN的活性方面至关重要。在这种情况下，它显着影响了一系列的嗅觉驱动的行为中央嗜热宿主的偏好，这是至关重要的矢量能力的一个。coluzzii是人类疟疾的主要传播媒介。
Mosquitoes rely heavily on their olfactory systems for host seeking, selection of oviposition sites, and avoiding predators and other environmental dangers. Of these behaviors, the preferential selection of a human blood-meal host drives the vectorial capacity of anthropophilic female Anopheles coluzzii mosquitoes. Olfactory receptor neurons (ORNs) are dispersed across several appendages on the head and express an obligate odorant receptor co-receptor (Orco) coupled with a “tuning” odorant receptor (OR) to form heteromeric, odor-gated ion channels in the membrane of these neurons. To examine the mechanistic and functional contributions of Orco/OR complexes to the chemosensory processes of An. coluzzii, we utilized CRISPR/Cas9 gene editing to create a line of homozygous, Orco-knockout, mutant mosquitoes. As expected, orco−/− ORNs across both adult and larval stages of An. coluzzii display significantly lower background activity and lack nearly all odor-evoked responses. In addition, blood-meal-seeking, adult female, orco−/− mutant mosquitoes exhibit severely reduced attraction to human- and non-human-derived odors while gravid females are significantly less responsive to established oviposition attractants. These results reinforce observations in other insects that Orco is crucial in maintaining the activity of ORNs. In that light, it significantly influences a range of olfactory-driven behaviors central to the anthropophilic host preference that is critical to the vectorial capacity of An. coluzzii as a primary vector for human malaria.
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DOI：
10.1111/j.1460-9568.2007.05786.x
发表时间：
2007-09
期刊：
The European journal of neuroscience
影响因子：
--
作者：
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通讯作者：
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影响因子：
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作者：
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发表时间：
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期刊：
Trends in genetics : TIG
影响因子：
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作者：
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影响因子：
2.2
作者：
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影响因子：
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