Bartonella Prevalence and Genome Sequences in Rodents in Some Regions of Xinjiang, China.
Bartonella Prevalence and Genome Sequences in Rodents in Some Regions of Xinjiang, China.
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DOI：
10.1128/aem.01964-22
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发表时间：
2023-04-26
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
本研究调查了新疆维吾尔自治区北屯鼠类巴尔通体感染及其遗传多样性。2018年，在四个采样点使用捕捉器捕获了小型哺乳动物。收集脾脏和肝脏组织培养分离巴尔通体菌株。对gltA基因PCR鉴定为巴尔通体的菌株进行全基因组测序，使用FastANI v1.33软件计算基因组的平均核苷酸同一性（ANI）。gltA PCR检测巴尔通体阳性样本，基于1290 bp gltA基因、2903 bp rpoB基因和核心基因组单核苷酸多态性（snp）构建系统发育树。66只鼠中巴尔通体阳性11只，感染率为16.67%。不同组织（χ2 = 2.133, P = 0.242）、不同种类（χ2 = 9.631, P = 0.141）、不同生境（χ2 = 4.309, P = 0.312）的鼠感染率均无统计学差异。从鼠中分离到的巴尔通体属在系统发育上分为6支（2只鼠中检出2种不同的巴尔通体）。通过比较基于gltA基因、rpoB基因和snp的系统发育树，我们发现几种进化树的拓扑结构是不同的。然而，本研究分离的巴尔通体菌株在不同的系统发育树上聚集成6个簇。新疆北屯鼠类巴尔通体分布广泛，遗传多样性高。重要性啮齿动物传播的巴尔通体与人畜共患疾病有关。巴尔通体种类，如伊丽莎白巴尔通体、格拉罕巴尔通体和三角巴尔通体可引起人类疾病。人类可通过被感染宿主的抓伤和咬伤或通过与传染性啮齿动物接触而被吸血节肢动物感染。新疆是中国巴尔通体种类最丰富的省份之一，但北屯地区巴尔通体流行情况报道较少。本研究旨在了解这些采样点鼠类巴尔通体感染的发生和流行情况，为北屯及新疆周边地区鼠类巴尔通体的防治提供依据。
In this study, we investigated Bartonella infection and its genetic diversity in rodents in Beitun, Xinjiang Uygur Autonomous Region, China. Small mammals were captured using snap traps at four sampling sites in 2018. Spleen and liver tissues were collected and cultured to isolate Bartonella strains. Whole-genome sequencing was performed on the strains identified as Bartonella by gltA gene PCR, and the average nucleotide identity (ANI) of the genomes was calculated by using FastANI v1.33. Phylogenetic trees were constructed for the samples positive for Bartonella spp. by the gltA PCR assay based on 1,290-bp gltA genes, 2,903-bp rpoB genes, and core-genome single nucleotide polymorphisms (SNPs). Among 66 rodents, 11 were positive for Bartonella, with an infection rate of 16.67%. The rodent infection rates in different tissues (χ2 = 2.133; P = 0.242), species (χ2 = 9.631; P = 0.141), and habitats (χ2 = 4.309; P = 0.312) did not show statistical differences. Bartonella spp. isolated from the rodents were phylogenetically divided into six clades (two different Bartonella species were detected in two rodents). By comparing phylogenetic trees based on gltA genes, rpoB genes, and SNPs, we found that the topological structures of several evolutionary trees are different. However, the Bartonella strains isolated in this study were clustered into six clusters in different phylogenetic trees. Broad distributions and high genetic diversity of Bartonella strains were observed among rodents in Beitun, Xinjiang. IMPORTANCE Rodent-borne Bartonella species have been associated with zoonotic diseases. Bartonella species such as Bartonella elizabethae, Bartonella grahamii, and Bartonella tribocorum can cause disease in humans. Humans can be infected by blood-sucking arthropods through the scratches and bites of an infected reservoir host or via contact with infectious rodents. Xinjiang is one of the provinces with the most abundant species of Bartonella in China, but there are few reports about the prevalence of Bartonella in the Beitun area. This research aims to investigate the occurrence and prevalence of Bartonella infection in rodents at these sampling sites and provide a basis for the prevention and control of rodent Bartonella species in Beitun and the surrounding areas of Xinjiang.
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影响因子：
4.5
作者：
通讯作者：
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影响因子：
9.4
作者：
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通讯作者：
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DOI：
10.4269/ajtmh.2010.09-0778
发表时间：
2010-06-01
影响因子：
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