TPF imaging of Rhodamine B at different detection windows

TPF imaging of Rhodamine B at different detection windows
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不同检测窗口下罗丹明 B 的 TPF 成像

DOI:
10.1142/s0217984919502683
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发表时间:
2019-08
影响因子:
1.9
通讯作者:
Xia Yuanqin
Xia Yuanqin
中科院分区:
物理与天体物理4区
文献类型:
--
作者:
Hon Guozhong;Dong Zhiwei;Zhang Sheng;Zhang Zhibin;Xia Yuanqin

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建立了基于飞秒振荡器的宽带双光子荧光成像系统。在不同的探测窗口下,对罗丹明B分子产生的宽带TPF信号进行了探测。在545-615 nm的短波长检测窗口和642-708 nm的长波长检测窗口处检测到的TPF信号都用于TPF成像。通过将探测窗口从短波长切换到长波长,实现了成像深度的加深。TPF成像结果表明,该成像系统具有较高的成像性能和良好的可靠性,单像素帧成像时间为4-8 s。
Broadband two-photon fluorescence (TPF) imaging system based on femtosecond oscillator is built. Broadband TPF signal generated from Rhodamine B molecule is detected at different detection windows. Both TPF signals detected at short wavelength detection window of 545–615 nm and long wavelength detection window of 642–708 nm are used for TPF imaging. Through switching detection window from short wavelength to long wavelength, deepened imaging depth is achieved. The TPF imaging results, with single [Formula: see text] pixels frame taking time of 4–8 s, demonstrate high performance and the good reliability of the imaging system.
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