The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila.
The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila.
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DOI：
10.1371/journal.pone.0032302
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Nakagoshi H
中科院分区：
文献类型：
--
作者：
Minami R;Wakabayashi M;Sugimori S;Taniguchi K;Kokuryo A;Imano T;Adachi-Yamada T;Watanabe N;Nakagoshi H
果蝇雄性附腺具有与哺乳动物前列腺和精囊腺相似的功能，并将附腺蛋白分泌到精液中。附腺的两个叶中的每一个都由两种类型的双核细胞组成：约1，000个主细胞和40个次级细胞。一个众所周知的附属腺蛋白，性肽，从主细胞分泌，并诱导雌性交配后反应，以增加后代生产，而鲜为人知的生理意义的次级细胞。同源结构域转录抑制因子缺陷前胃（Dve）在成体次级细胞中强烈表达，其突变导致次级细胞丢失，主细胞单核化，以及副腺大小减小。尽管存在性肽，dve突变体雄性仍具有低生殖力，并且不能诱导雌性交配后的反应，即产卵增加和性感受性降低。RNAi介导的dve敲低雄性也具有正常双核主细胞的低生殖力。我们提供的第一个证据表明，次级细胞是男性生殖力的关键，也是Dve活动所需的次级细胞的生存。这些发现为生育力/生殖力机制提供了新的见解。
The Drosophila male accessory gland has functions similar to those of the mammalian prostate gland and the seminal vesicle, and secretes accessory gland proteins into the seminal fluid. Each of the two lobes of the accessory gland is composed of two types of binucleate cell: about 1,000 main cells and 40 secondary cells. A well-known accessory gland protein, sex peptide, is secreted from the main cells and induces female postmating response to increase progeny production, whereas little is known about physiological significance of the secondary cells. The homeodomain transcriptional repressor Defective proventriculus (Dve) is strongly expressed in adult secondary cells, and its mutation resulted in loss of secondary cells, mononucleation of main cells, and reduced size of the accessory gland. dve mutant males had low fecundity despite the presence of sex peptide, and failed to induce the female postmating responses of increased egg laying and reduced sexual receptivity. RNAi-mediated dve knockdown males also had low fecundity with normally binucleate main cells. We provide the first evidence that secondary cells are crucial for male fecundity, and also that Dve activity is required for survival of the secondary cells. These findings provide new insights into a mechanism of fertility/fecundity.
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DOI：
10.1073/pnas.1631700100
发表时间：
2003-08-19
影响因子：
11.1
作者：
Liu, HF;Kubli, E
通讯作者：
Kubli, E
影响因子：
14.9
作者：
Gong F;Sun L;Wang Z;Shi J;Li W;Wang S;Han X;Sun Y
通讯作者：
Sun Y
影响因子：
2.4
作者：
Kosman, D;Small, S;Reinitz, J
通讯作者：
Reinitz, J
影响因子：
2.6
作者：
Nakagawa, Yoshiki;Fujiwara-Fukuta, Shinobu;Nakagoshi, Hideki
通讯作者：
Nakagoshi, Hideki
影响因子：
2.6
作者：
BERTRAM, MJ;AKERKAR, GA;WOLFNER, MF
通讯作者：
WOLFNER, MF