The study of effects of pore architecture in chitosan scaffolds on the fluid flow pattern by Doppler OCT
The study of effects of pore architecture in chitosan scaffolds on the fluid flow pattern by Doppler OCT
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多普勒 OCT 研究壳聚糖支架孔隙结构对流体流动模式的影响
DOI：
10.1117/12.842266
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Yang Y
中科院分区：
文献类型：
--
作者：
Yang Y
对组织工程中动态培养条件的优化和充分认识，可以加速这一新技术在医学领域的应用。组织工程中使用的支架通常具有高度多孔性，根据制造它们的技术不同，具有不同的孔隙结构。通过生物反应器中的支架灌注培养液已被证明可以有效地增强细胞支架结构内营养物质和气体的交换。灌注后，孔隙中流动的流体不可避免地在孔隙壁上产生剪切应力，这反过来又会引起具有机械换能器的细胞的细胞反应。因此，在三维细胞培养环境中建立灌注速率、流体剪切应力和孔隙结构之间的关系是组织工程师面临的一项具有挑战性的任务，因为相同的入口流速会引起孔隙内流速的局部变化。直到最近，还没有合适的非破坏性监测技术能够测量不透明厚物体的流速。在本研究中，壳聚糖支架通过冷冻干燥或孔隙浸出或碱性凝胶技术制备了孔隙结构改变的壳聚糖支架。多普勒光学相干断层扫描（DOCT）被用于区分具有细长孔结构和均匀圆孔结构的支架内的流速模式。获得了支架的结构和流动图像。研究发现，在给定进口流量下，孔隙连通性对于获得稳定流动至关重要。此外，不同的内部孔隙结构对局部流速模式也有影响。
Optimizing and fully understanding the dynamic culture conditions in tissue engineering could accelerate exploration of this new technique into a promising therapy in the medical field. Scaffolds used in tissue engineering usually are highly porous with various pore architecture depending on techniques that manufacture them. Perfusing culture fluid through a scaffold in a bioreactor has proven efficient in enhancing the exchange of nutrients and gas within cell-scaffold constructs. Upon perfusion, flowing fluid in pores inevitably produces shear stress on the wall of the pores, which will in turn induce cellular response for the cells possessing mechanotransducers. Thus, establishing a relationship between perfusion rate, fluid shear stress and pore architecture in a 3-dimensional cell culture environment is a challenging task faced by tissue engineers because the same inlet flow rate could induce local variation of flow rate within the pores. Until recently, there is no proper non-destructive monitoring technique available that is capable of measuring flow rate in opaque thick objects. In this study, chitosan scaffolds with altered pore architectures were manufactured by freeze-drying or porogen leaching out or alkaline gelation techniques. Doppler optical coherence tomography (DOCT) has been used to differentiate the flow rate pattern within scaffolds which have either elongating pore structure or homogeneous round pore structure. The structural and flow images have been obtained for the scaffolds. It is found that pore interconnectivity is critically important in obtaining a steady flow under a given inlet flow rate. In addition, different internal pore structures affect local flow rate pattern.
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影响因子：
3.5
作者：
Jia Y;Bagnaninchi PO;Yang Y;Haj AE;Hinds MT;Kirkpatrick SJ;Wang RK
通讯作者：
Wang RK
通过傅里叶域多普勒光学相干断层扫描表征 3D 多孔结构中的局部流体流动
DOI：
--
发表时间：
2007
期刊：
SPIE BiOS
影响因子：
--
作者：
P. Bagnaninchi;Y. Yang;A. E. El Haj;M. Hinds;R. Wang
通讯作者：
R. Wang
多普勒光学微血管造影改善了 3D 多孔结构内局部流体流动和剪切应力的量化。
影响因子：
3.5
作者：
Jia,Yali;An,Lin;Wang,RuikangK
通讯作者：
Wang,RuikangK
使用光学相干断层扫描和偏振敏感光学相干断层扫描表征支架结构和肌腱
DOI：
--
发表时间：
2008
期刊：
影响因子：
--
作者：
Ying Yang;P. Bagnaninchi;Bin Hu;K. Hampson;A. E. Haj
通讯作者：
A. E. Haj
流体力学基础：Philip M. Gerhart 和 Richard J. Gross 着，Addison Wesley，1985 年。ISBN 0-201-11410-0，856 页，插图，精装本，39.95 英镑
DOI：
--
发表时间：
1988
期刊：
影响因子：
--
作者：
T. Al
通讯作者：
T. Al