Polygonatum odoratum Polysaccharides Modulate Gut Microbiota and Mitigate Experimentally Induced Obesity in Rats.
Polygonatum odoratum Polysaccharides Modulate Gut Microbiota and Mitigate Experimentally Induced Obesity in Rats.
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DOI：
10.3390/ijms19113587
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发表时间：
2018-11-13
影响因子：
5.6
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Wang Y;Fei Y;Liu L;Xiao Y;Pang Y;Kang J;Wang Z
越来越多的证据表明，肠道微生物群在代谢性疾病中起着至关重要的作用。玉竹提取物具有降血糖和降血脂作用，但其作用机制尚不清楚。本研究探讨了飞机草多糖（POP）对高脂饮食（HFD）诱导的大鼠肥胖的影响，以及这些影响是否与肠道微生物群的调节有关。POP治疗减弱了HFD喂养大鼠的体重增加、脂肪蓄积、附睾脂肪细胞大小、肝脏甘油三酯和肝脏总胆固醇含量。POP给药还增加了短链脂肪酸（SCFA），包括异丁酸、丁酸和戊酸。POP可上调脂肪细胞分化相关基因（Pparg、Cebpa、Cebpb）和脂解相关基因（Ppara、Atgl）的表达，下调脂质合成相关基因（Srebpf1、Fabp4、Fas）的表达，并导致相应的PPARγ和FABP4蛋白表达的改变。最后，持久性有机污染物提高了物种丰富度，改善了肠道微生物群的群落结构，减少了梭菌，肠球菌，粪杆菌，乳球菌和Sutterella的相对丰度。主坐标分析（PCoA）显示HFD喂养大鼠与所有其他给药组之间存在明显分离。相关性分析确定了肥胖表型和28个受POP影响的操作分类单位（OTU）之间的负相关和正相关，包括推定的SCFA产生细菌。我们的数据表明，POP补充剂可能会减弱与肠道微生物群调节相关的HFD喂养大鼠的肥胖特征。
Increasing evidence suggests that the gut microbiota plays vital roles in metabolic diseases. Polygonatum odoratum extract alleviates hyperglycemia and hyperlipidemia, but the underlying mechanism remains unclear. This study investigated the effects of P. odoratum polysaccharides (POPs) on high-fat diet (HFD)-induced obesity in rats and whether these effects were related to modulation of gut microbiota. POP treatment attenuated weight gain, fat accumulation, epididymal adipocyte size, liver triglycerides, and total liver cholesterol content in HFD-fed rats. POP administration also increased short-chain fatty acids (SCFAs), including isobutyric acid, butyric acid, and valeric acid. POP upregulated the expression of genes involved in adipocyte differentiation (Pparg, Cebpa, Cebpb) and lipolysis (Ppara, Atgl), and downregulated those related to lipid synthesis (Srebpf1, Fabp4, Fas), with corresponding changes in PPARγ and FABP4 protein expression. Finally, POP enhanced species richness and improved the gut microbiota community structure, reducing the relative abundances of Clostridium, Enterococcus, Coprobacillus, Lactococcus, and Sutterella. Principal coordinates analysis (PCoA) revealed a clear separation between HFD-fed rats and all other treatment groups. Correlation analysis identified negative and positive associations between obesity phenotypes and 28 POP-influenced operational taxonomic units (OTUs), including putative SCFA-producing bacteria. Our data suggest that POP supplementation may attenuate features of obesity in HFD-fed rats in association with the modulation of gut microbiota.
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影响因子：
7.7
作者：
Garin-Shkolnik, Tali;Rudich, Assaf;Rubinstein, Menachem
通讯作者：
Rubinstein, Menachem
影响因子：
3.7
作者：
Cui C;Li Y;Gao H;Zhang H;Han J;Zhang D;Li Y;Zhou J;Lu C;Su X
通讯作者：
Su X
影响因子：
15.5
作者：
Fadrosh DW;Ma B;Gajer P;Sengamalay N;Ott S;Brotman RM;Ravel J
通讯作者：
Ravel J
影响因子：
2.9
作者：
Chandler, PC;Viana, JB;Boggiano, MM
通讯作者：
Boggiano, MM
DOI：
10.1016/j.ijbiomac.2012.12.010
发表时间：
2013-03-01
影响因子：
8.2
作者：
Jiang, Qunguang;Lv, Yunxia;Zhong, Dewu
通讯作者：
Zhong, Dewu