Effect of fiber insertion depth on antibacterial efficacy of photodynamic therapy against Enterococcus faecalis in rootcanals
Effect of fiber insertion depth on antibacterial efficacy of photodynamic therapy against Enterococcus faecalis in rootcanals
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纤维插入深度对光动力治疗根管内粪肠球菌抗菌效果的影响
DOI：
10.1007/s00784-016-1948-3
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发表时间：
2017
影响因子：
3.4
通讯作者：
Annette
中科院分区：
文献类型：
--
作者：
Rödig;Endres;Konietschke;Zimmermann;Ortrud;Hans Georg;Wiegand;Annette
目的本体外研究评估了纤维插入深度对感染粪肠球菌根管中使用光敏剂（PS；甲苯胺蓝）和红色发光二极管（LED）的抗菌光动力疗法（aPDT）抗菌效果的影响。材料和方法用镍钛器械制备单根拔牙，消毒，污染大肠杆菌。 faecalis，并孵育 72 小时。将根随机分为四个实验组：仅 PS、仅 LED、aPDT 在顶端三分之一处具有 LED、aPDT 在冠状三分之一处具有 LED，以及感染和无菌对照（每组 = 10）。通过从根管壁收集标准化牙本质刨花来获取样本。在血琼脂上连续稀释和培养后，对菌落形成单位（CFU）进行计数。结果与感染对照相比，两个aPDT组的CFU均减少了1-2 log10steps，而纤维插入深度的影响可以忽略不计（<0.5 log10steps）。与感染对照相比，单独使用 PS 的 CFU 减少了大约 0.5 log10steps，但单独使用 PS 的效果不如两个 aPDT 组。单独使用 LED 没有检测到抗菌作用。 结论 aPDT 降低了 E。根管内的粪肠球菌，而纤维插入深度对aPDT抗菌效果的影响可以忽略不计。临床相关性发光二极管的插入深度可能不会影响光动力疗法对大肠杆菌的抗菌效果。粪菌素直根管。
ObjectivesThis in vitro study evaluated the effect of fiber insertion depth on antimicrobial efficacy of antimicrobial photodynamic therapy (aPDT) using a photosensitizer (PS; toluidine blue) and a red light-emitting diode (LED) in root canals infected withEnterococcus faecalis.Materials and methodsSingle-rooted extracted teeth were prepared with nickel-titanium-instruments, sterilized, contaminated withE. faecalis, and incubated for 72 h. Roots were randomly divided into four experimental groups: PS only, LED only, aPDT with LED in the apical third, aPDT with LED in the coronal third, as well as into infection and sterile controls (eachn= 10). Samples were taken by collecting standardized dentine shavings from the root canal walls. After serial dilution and culturing on blood agar, colony-forming units (CFU) were counted.ResultsBoth aPDT groups showed a CFU reduction of 1–2 log10steps compared with the infection control, whereas the effect of fiber insertion depth was negligible (<0.5 log10steps). CFU reduction of approximately 0.5 log10steps for PS alone was detected compared with the infection control, but PS alone was less effective than both aPDT groups. No antibacterial effect was detected for LED alone.ConclusionsaPDT reducedE. faecaliswithin the root canal, whereas fiber insertion depth had a negligible influence on antimicrobial effectiveness of aPDT.Clinical relevanceThe insertion depth of the light-emitting diode may not influence the antibacterial efficacy of photodynamic therapy againstE. faecalisin straight root canals.
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影响因子：
4.2
作者：
Garcez, Aguinaldo S.;Nunez, Silvia C.;Ribeiro, Martha S.
通讯作者：
Ribeiro, Martha S.
影响因子：
4.2
作者：
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Siqueira, Jose F., Jr.
影响因子：
2.6
作者：
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通讯作者：
Pearson, GJ
影响因子：
3.4
作者：
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影响因子：
5
作者：
T. M. Odor;Nicholas P. Chandler;T. Watson;T. Ford;Fraser McDonald
通讯作者：
Fraser McDonald