Removing the sporoderm from the sporoderm-broken spores of Ganoderma lucidum improves the anticancer and immune-regulatory activity of the water-soluble polysaccharide.
Removing the sporoderm from the sporoderm-broken spores of Ganoderma lucidum improves the anticancer and immune-regulatory activity of the water-soluble polysaccharide.
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将灵芝破壁孢子中的孢子皮去除，提高了水溶性多糖的抗癌和免疫调节活性。
DOI：
10.3389/fnut.2022.1006127
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发表时间：
2022
影响因子：
5
通讯作者：
Wang, Xingya
中科院分区：
文献类型：
--
作者：
Fang, Liu;Zhao, Qian;Guo, Cuiling;Guo, Dandan;Li, Zhenhao;Xu, Jing;Guo, Chengjie;Sang, Tingting;Wang, Ying;Chen, Jiajun;Chen, Chaojie;Chen, Rong;Wu, Jianjun;Wang, Xingya
植物来源的多糖已被证明通过免疫调节活性具有很好的抗癌作用。比较药用真菌灵芝（Ganoderma lucidum，RSGLP）去孢壁孢子和灵芝破壁孢子多糖的化学性质和抗肿瘤作用。灵芝（BSGLP）。我们发现RSGLP比BSGLP具有更高的提取率。HPGPC和GC-MS结果表明，RSGLP和BSGLP均为杂多糖，但RSGLP的分子量较大，单糖组成比例不同。MTT和流式细胞术结果表明，RSGLP在抑制细胞活力和诱导细胞凋亡方面表现出比BSGLP高得多的剂量-功效，在代表结肠癌（HCT 116和HT 29）、肝癌（HepG 2和Huh-7）、乳腺癌（MDA-MB-231和MCF-7）和肺癌（NCI-H460和A549）的8种癌细胞系中。RSGLP对裸鼠移植瘤HCT 116和NCI-H460生长的抑制作用及对脾肿大的抑制作用均优于BSGLP，提示RSGLP具有更好的免疫调节作用。在体外实验中，RSGLP对巨噬细胞RAW 264. 7的活化及炎症介质IL-1β、TNF-α、iNOS、考克斯-2的表达有较强的抑制作用。这是第一项使用多种癌细胞系比较RSGLP和BSGLP的化学性质，抗癌和免疫调节作用的研究。结果表明，去孢壁的G.灵芝多糖（RSGL）和RSGLP具有良好的免疫调节活性，有望成为新的抗癌药物。
Plant-derived polysaccharides have demonstrated promising anti-cancer effects via immune-regulatory activity. The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP). We found that RSGLP has a higher extraction yield than BSGLP. HPGPC and GC-MS results revealed that both RSGLP and BSGLP are heteropolysaccharides, but RSGLP had a higher molecular weight and a different ratio of monosaccharide composition than BSGLP. MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549). Furthermore, RSGLP is more effective in inhibiting HCT116 and NCI-H460 xenograft tumor growth and inhibiting tumor-induced splenomegaly than BSGLP in nude mice, suggesting a better effect on regulating immunity of RSGLP. Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro. This is the first study to compare the chemical properties, anti-cancer, and immune-regulatory effects of RSGLP and BSGLP using multiple cancer cell lines. Our results revealed that the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP may serve as new anticancer agents for their promising immune-regulatory activity.
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影响因子：
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作者：
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