Intra-operatively obtained human tissue: protocols and techniques for the study of neural stem cells.
Intra-operatively obtained human tissue: protocols and techniques for the study of neural stem cells.
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DOI：
10.1016/j.jneumeth.2009.02.014
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发表时间：
2009-05-30
影响因子：
3
通讯作者：
Quiñones-Hinojosa A
中科院分区：
文献类型：
--
作者：
Chaichana KL;Guerrero-Cazares H;Capilla-Gonzalez V;Zamora-Berridi G;Achanta P;Gonzalez-Perez O;Jallo GI;Garcia-Verdugo JM;Quiñones-Hinojosa A
神经干细胞（NSC）和脑肿瘤干细胞（BTSC）分别在成人大脑和脑肿瘤中的发现，开创了神经科学研究的新时代。这些细胞代表了研究记忆和学习等正常现象以及帕金森病、中风和脑肿瘤等病理状况的新方法。这种新的模式强调了了解这些细胞在体外和体内如何表现的重要性。它还强调需要使用人源性组织来研究人类疾病，因为动物模型可能不一定准确地复制人类中发生的过程。一个重要的，但往往未得到充分利用，人类组织的来源，因此，神经干细胞和BTSC，是手术室。本研究详细描述了当前和新开发的实验室技术，根据我们的经验，这些技术用于处理和研究直接从手术室获得的组织中的人类NSC和BTSC。
The discoveries of neural (NSCs) and brain tumor stem cells (BTSCs) in the adult human brain and in brain tumors, respectively, have led to a new era in neuroscience research. These cells represent novel approaches to studying normal phenomena such as memory and learning, as well as pathological conditions such as Parkinson’s disease, stroke, and brain tumors. This new paradigm stresses the importance of understanding how these cells behave in vitro and in vivo. It also stresses the need to use human-derived tissue to study human disease because animal models may not necessarily accurately replicate the processes that occur in humans. An important, but often underused, source of human tissue and, consequently, both NSCs and BTSCs, is the operating room. This study describes in detail both current and newly developed laboratory techniques, which in our experience are used to process and study human NSCs and BTSCs from tissue obtained directly from the operating room.
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影响因子：
82.9
作者：
Eriksson, PS;Perfilieva, E;Gage, FH
通讯作者：
Gage, FH
DOI：
10.1073/pnas.250471697
发表时间：
2000-12-05
影响因子：
11.1
作者：
Laywell, ED;Rakic, P;Steindler, DA
通讯作者：
Steindler, DA
影响因子：
11.2
作者：
Galli, R;Binda, E;Vescovi, A
通讯作者：
Vescovi, A
影响因子：
5.2
作者：
Copray, Sjef;Balasubramaniyan, Veerakumar;Boddeke, Erik
通讯作者：
Boddeke, Erik
DOI：
10.1073/pnas.95.6.3168
发表时间：
1998-03-17
影响因子：
11.1
作者：
Gould, E;Tanapat, P;Fuchs, E
通讯作者：
Fuchs, E