Seroepidemiology of human polyomaviruses.
Seroepidemiology of human polyomaviruses.
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DOI：
10.1371/journal.ppat.1000363
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发表时间：
2009-03
期刊：
影响因子：
6.7
通讯作者：
Garcea RL
中科院分区：
文献类型：
--
作者：
Kean JM;Rao S;Wang M;Garcea RL
除了先前表征的病毒BK和JC之外，最近还鉴定了三种新的人多瘤病毒（Pys）：KIV、WUV和默克尔细胞Py（MCV）。使用采用重组VP 1衣壳蛋白的ELISA，我们测定了KIV、WUV和MCV、沿着BKV和JCV以及猴病毒SV 40和LPV的血清阳性率。可溶性VP 1蛋白用于评估病毒之间的交叉反应性。我们发现健康成人献血者（1501）的血清阳性率（+/− 1%）为SV 40（9%）、BKV（82%）、JCV（39%）、LPV（15%）、KIV（55%）、WUV（69%）、MCV毒株350（25%）和MCV毒株339（42%）。竞争试验检测到LPV或MCV的VP 1蛋白之间或WUV和KIV之间没有血清交叉反应性。SV 40和BKV之间有相当大的血清交叉反应性，在较小程度上，SV 40和JCV VP 1蛋白之间也有交叉反应性。校正交叉反应性后，SV 40血清阳性率为1.2%。所有Pys在21岁以下儿童（n =721）中的血清阳性率与成人人群相似，表明这些病毒的主要暴露可能发生在儿童时期。 多瘤病毒在从鸟类到人类的动物中占据复制生态位。两种人类多瘤病毒BKV和JCV于1971年被发现，并且在过去的两年中，在人类中发现了三种新的多瘤病毒：KI（KIV）、WU（WUV）和默克尔细胞（MCV）多瘤病毒。在默克尔细胞癌（一种罕见的皮肤癌）中发现了MCV。到目前为止，还没有确定人口中有多少百分比接触了KIV、WUV和MCV，以及何时开始接触这些病毒。我们确定KIV，WUV和MCV的初始暴露发生在儿童时期，类似于已知的人类多瘤病毒BKV和JCV，并且它们的流行率很高。我们还发现证据表明，猴病毒嗜酸性多瘤病毒（LPV）可能具有血清学相关的人类对应物。另一种猴多瘤病毒SV 40的流行率为0.2%，这一水平不支持其在人类疾病中的作用。
In addition to the previously characterized viruses BK and JC, three new human polyomaviruses (Pys) have been recently identified: KIV, WUV, and Merkel Cell Py (MCV). Using an ELISA employing recombinant VP1 capsid proteins, we have determined the seroprevalence of KIV, WUV, and MCV, along with BKV and JCV, and the monkey viruses SV40 and LPV. Soluble VP1 proteins were used to assess crossreactivity between viruses. We found the seroprevalence (+/− 1%) in healthy adult blood donors (1501) was SV40 (9%), BKV (82%), JCV (39%), LPV (15%), KIV (55%), WUV (69%), MCV strain 350 (25%), and MCV strain 339 (42%). Competition assays detected no sero-crossreactivity between the VP1 proteins of LPV or MCV or between WUV and KIV. There was considerable sero-crossreactivity between SV40 and BKV, and to a lesser extent, between SV40 and JCV VP1 proteins. After correcting for crossreactivity, the SV40 seroprevalence was ∼2%. The seroprevalence in children under 21 years of age (n = 721) for all Pys was similar to that of the adult population, suggesting that primary exposure to these viruses likely occurs in childhood. Polyomaviruses occupy a replicative niche in animals from birds to humans. Two human polyomaviruses, BKV and JCV, were discovered in 1971 and within the last two years, three new polyomaviruses have been found in humans: KI (KIV), WU (WUV), and Merkel Cell (MCV) polyomavirus. MCV was identified in Merkel Cell carcinomas, a rare skin cancer. To date, it has not been determined what percentage of the human population is exposed to KIV, WUV, and MCV, and when initial exposure to these viruses occurs. We determined that initial exposure to KIV, WUV, and MCV occurs in childhood, similar to that for the known human polyomaviruses BKV and JCV, and that their prevalence is high. We also found evidence that a monkey virus, Lymphotropic Polyomavirus (LPV), likely has a serologically related human counterpart. Another monkey polyomavirus, SV40, was found at ∼2% prevalence, a level that does not support its role in human disease.
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影响因子：
64.8
作者：
PALMITER, RD;CHEN, HY;BRINSTER, RL
通讯作者：
BRINSTER, RL
影响因子：
8.8
作者：
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通讯作者：
WALKER, DL
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作者：
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通讯作者：
Sloot, Theo P.