Sub-chronic oral toxicity screening of quercetin in mice.
Sub-chronic oral toxicity screening of quercetin in mice.
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DOI：
10.1186/s12906-022-03758-z
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发表时间：
2022-10-23
影响因子：
3.9
通讯作者：
Velazquez, Kandy T.
中科院分区：
文献类型：
--
作者：
Cunningham, Patrice;Patton, Emma;VanderVeen, Brandon N.;Unger, Christian;Aladhami, Ahmed;Enos, Reilly T.;Madero, Sarah;Chatzistamou, Ioulia;Fan, Daping;Murphy, E. Angela;Velazquez, Kandy T.
槲皮素是一种有机类黄酮，存在于几种水果和蔬菜中。这种膳食补充剂所证明的抗炎、抗病毒、抗氧化、保护心脏、抗癌和保护神经的特性，使其成为治疗炎症性疾病和癌症的可能方法。不幸的是，相互矛盾的研究给槲皮素的毒性带来了不确定性。这项研究的主要目的是确定槲皮素在临床前研究中是否对小鼠有毒性作用在癌症，癌症治疗和脱靶效应方面，槲皮素的剂量已经显示出效力。研究了槲皮素对雄性和雌性CD2F1小鼠的亚慢性毒性。将三种不同剂量的槲皮素（62、125和250 mg/kg日粮）灌注到AIN-76A纯化日粮中，给药98天。在实验过程的不同时间点评估体重（BW）、食物消耗量、饮水量、身体组成、血细胞计数、行为和代谢表型。观察组织脏器大体病理变化，血浆测定碱性磷酸酶（AP）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）。我们发现，喂食低（62 mg/kg）、中（125 mg/kg）和高（250 mg/kg）的槲皮素对身体组成、器官功能、行为或代谢没有明显的影响。总之，我们的研究表明，槲皮素在雌性和雄性CD2F1小鼠中是安全的，每天给药12.5、25或50 mg/kg体重，持续14周（即98天）。需要进行进一步的研究以确定长期摄入槲皮素后的任何潜在毒性。
Quercetin is an organic flavonoid present in several fruits and vegetables. The anti-inflammatory, antiviral, antioxidant, cardio-protective, anti-carcinogenic and neuroprotective properties demonstrated by this dietary supplement endorses it as a possible treatment for inflammatory diseases and cancer. Unfortunately, conflicting research has cast uncertainties on the toxicity of quercetin. The main purpose of this study was to determine if quercetin has any toxic properties in mice at doses that have shown efficacy in pre-clinical studies regarding cancer, cancer therapy, and their off-target effects. A sub-chronic toxicity study of quercetin was examined in male and female CD2F1 mice. Three different doses of quercetin (62, 125, and 250 mg/kg of diet) were infused into the AIN-76A purified diet and administered to mice ad libitum for 98 days. Body weight (BW), food consumption, water intake, body composition, blood count, behavior, and metabolic phenotype were assessed at various timepoints during the course of the experiment. Tissue and organs were evaluated for gross pathological changes and plasma was used to measure alkaline phosphatase (AP), aspartate transaminase (AST), and alanine transaminase (ALT). We found that low (62 mg/kg of diet), medium (125 mg/kg of diet), and high (250 mg/kg of diet) quercetin feeding had no discernible effect on body composition, organ function, behavior or metabolism. In summary, our study establishes that quercetin is safe for use in both female and male CD2F1 mice when given at ~ 12.5, 25, or 50 mg/kg of BW daily doses for 14 weeks (i.e. 98 days). Further studies will need to be conducted to determine any potential toxicity of quercetin following chronic ingestion.
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影响因子：
2.9
作者：
Mahoney SE;Davis JM;Murphy EA;McClellan JL;Pena MM
通讯作者：
Pena MM
影响因子：
9.8
作者：
Stewart, Laura K.;Soileau, Jeff L.;Ribnicky, David;Wang, Zhong Q.;Raskin, Ilya;Poulev, Alexander;Majewski, Martin;Cefalu, William T.;Gettys, Thomas W.
通讯作者：
Gettys, Thomas W.
DOI：
10.1152/ajpregu.90319.2008
发表时间：
2008-08-01
影响因子：
2.8
作者：
Davis, J. M.;Murphy, E. A.;Gangemi, J. D.
通讯作者：
Gangemi, J. D.
影响因子：
9.7
作者：
HIRONO, I;UENO, I;NATORI, S
通讯作者：
NATORI, S
影响因子：
3.6
作者：
Steiner, J. L.;Davis, J. M.;Murphy, E. A.
通讯作者：
Murphy, E. A.