Alcohol exposure inhibits adult neural stem cell proliferation.
Alcohol exposure inhibits adult neural stem cell proliferation.
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酒精暴露会抑制成人神经干细胞的增殖。
DOI：
10.1007/s00221-014-3958-1
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发表时间：
2014-09
影响因子：
2
通讯作者：
Kippin, Tod E.
中科院分区：
文献类型：
--
作者：
Campbell, Joannalee C.;Stipcevic, Tamara;Flores, Roberto E.;Perry, Canelda;Kippin, Tod E.
酒精暴露可以减少成人的增殖和/或神经发生，但它对最终的神经源前体-神经干细胞(NSCs)的影响一直没有得到很好的解决。因此，本研究检测了自愿饮酒对侧脑室下区(SVZ)神经干细胞的影响。成年雄性C57BL/6J小鼠在自愿酒精暴露后，使用神经球试验在两瓶选择任务中测量神经干细胞数量，而在组织收集前两周已经增殖的神经干细胞数量使用溴脱氧尿嘧啶核苷(BrdU)保留来索引。与对照组相比，饮酒小鼠体内BrdU保留细胞的数量显著减少，但与对照组相比，饮酒小鼠SVZ中可衍生的神经球形成细胞的数量没有差异。此外，酒精暴露后，SVZ内的增殖细胞核抗原标记细胞数趋于减少，而齿状回内的BrdU标记细胞数无明显差异。为了确定酒精对神经干细胞及其后代的直接影响，在体外用酒精处理来自幼稚小鼠的神经球。100 mM酒精可减少神经球的形成，但不会降低细胞活力。这些发现首次评估了适度的自愿饮酒对成年神经干细胞选择性测量的影响，并表明这种暴露改变了体内NSC的增殖动力学，酒精对NSCs及其后代的体外扩增和存活有直接但可分离的影响。
Alcohol exposure can reduce adult proliferation and/or neurogenesis, but its impact on the ultimate neurogenic precursors, neural stem cells (NSCs), has been poorly addressed. Accordingly, the impact of voluntary consumption of alcohol on NSCs in the subventricular zone (SVZ) of the lateral ventricle was examined in this study. The NSC population in adult male C57BL/6J mice was measured after voluntary alcohol exposure in a two-bottle choice task using the neurosphere assay, while the number of NSCs that had proliferated two weeks prior to tissue collection was indexed using bromodeoxyuridine (BrdU) retention. There was a significant decrease in the number of BrdU-retaining cells in alcohol consuming mice compared to controls, but no difference in the number of neurosphere-forming cells that could be derived from the SVZ of alcohol consuming mice compared to controls. Additionally, PCNA-labeled cells in the SVZ tended to be lower but there was no difference in BrdU labeling in the dentate gyrus following alcohol exposure. To determine alcohol’s direct impact on NSCs and their progeny, neurospheres derived from naïve mice were treated with alcohol in vitro. Neurosphere formation was reduced by 100 mM alcohol without reducing cell viability. These findings are the first to assess the impact of moderate voluntary alcohol consumption on selective measures of adult NSCs and indicate that such exposure alters NSC proliferation dynamics in vivo and alcohol has direct but dissociable effects on the expansion and viability on NSCs and their progeny in vitro.
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DOI：
10.1007/978-4-431-53945-2_10
发表时间：
2011-01-01
期刊：
NEUROGENESIS IN THE ADULT BRAIN II: CLINICAL IMPLICATIONS
影响因子：
--
作者：
Hitoshi, Seiji;Kippin, Tod;van der Kooy, Derek
通讯作者：
van der Kooy, Derek
影响因子：
3.3
作者：
Anderson ML;Nokia MS;Govindaraju KP;Shors TJ
通讯作者：
Shors TJ
影响因子：
5.2
作者：
Coles-Takabe, Brenda L. K.;Brain, Ian;Van der Kooy, Derek
通讯作者：
Van der Kooy, Derek
影响因子：
2.6
作者：
DUDEK, BC;UNDERWOOD, KA
通讯作者：
UNDERWOOD, KA
DOI：
10.1111/j.1530-0277.2009.01079.x
发表时间：
2010-01-01
影响因子：
3.2
作者：
Fein, George;Shimotsu, Ryan;Barakos, Jerome
通讯作者：
Barakos, Jerome