Identification of genes for engineering the male germline of Aedes aegypti and Ceratitis capitata.
Identification of genes for engineering the male germline of Aedes aegypti and Ceratitis capitata.
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DOI：
10.1186/s12864-016-3280-3
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发表时间：
2016-11-21
期刊：
影响因子：
4.4
通讯作者：
Alphey AL
中科院分区：
文献类型：
--
作者：
Sutton ER;Yu Y;Shimeld SM;White-Cooper H;Alphey AL
合成生物学方法是控制传播疾病和造成农业损害的害虫的有前景的新策略。这些策略需要特征化的模块化组件，可以指导效应序列的适当表达，其中跨物种保守的组件特别有用。这项研究的目的是确定基因，从中可以衍生出新的潜在成分，用于操纵两种主要害虫物种（埃及伊蚊和实蝇）的雄性种系。利用分阶段睾丸样本的 RNA-seq 数据，我们鉴定了几个具有睾丸特异性表达的候选基因，以及在合成对照构建体中使用其调控区域的合适表达时机。我们还开发了一种新颖的计算管道，用于从这些数据中识别具有睾丸特异性剪接的候选基因；使用选择性剪接是限制合成系统中表达的另一种方法。一些鉴定出的基因表现出睾丸特异性表达或剪接，这些表达或剪接在物种间是保守的。这些是构建开发特别有前途的候选者。在这项研究中，我们鉴定了一组具有睾丸特异性表达或剪接的基因。除了从基本生物学角度来看，这些发现还为开发合成系统以通过操纵雄性种系来控制重要害虫提供了基础。本文的在线版本 (doi:10.1186/s12864-016-3280-3) 包含补充材料，可供授权用户使用。
Synthetic biology approaches are promising new strategies for control of pest insects that transmit disease and cause agricultural damage. These strategies require characterised modular components that can direct appropriate expression of effector sequences, with components conserved across species being particularly useful. The goal of this study was to identify genes from which new potential components could be derived for manipulation of the male germline in two major pest species, the mosquito Aedes aegypti and the tephritid fruit fly Ceratitis capitata. Using RNA-seq data from staged testis samples, we identified several candidate genes with testis-specific expression and suitable expression timing for use of their regulatory regions in synthetic control constructs. We also developed a novel computational pipeline to identify candidate genes with testis-specific splicing from this data; use of alternative splicing is another method for restricting expression in synthetic systems. Some of the genes identified display testis-specific expression or splicing that is conserved across species; these are particularly promising candidates for construct development. In this study we have identified a set of genes with testis-specific expression or splicing. In addition to their interest from a basic biology perspective, these findings provide a basis from which to develop synthetic systems to control important pest insects via manipulation of the male germline. The online version of this article (doi:10.1186/s12864-016-3280-3) contains supplementary material, which is available to authorized users.
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影响因子：
48
作者：
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通讯作者：
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影响因子：
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作者：
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Alphey L
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作者：
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通讯作者：
Alphey, Luke
DOI：
10.1073/pnas.1000251107
发表时间：
2010-03-09
影响因子：
11.1
作者：
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通讯作者：
Alphey, Luke