Female-specific flightless (fsRIDL) phenotype for control of Aedes albopictus.
Female-specific flightless (fsRIDL) phenotype for control of Aedes albopictus.
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DOI：
10.1371/journal.pntd.0001724
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发表时间：
2012
影响因子：
3.8
通讯作者：
Alphey L
中科院分区：
文献类型：
--
作者：
Labbé GM;Scaife S;Morgan SA;Curtis ZH;Alphey L
白纹伊蚊（亚洲虎蚊）是包括登革热和基孔肯雅热在内的多种虫媒病毒的载体，也是一种重要的滋扰蚊子。它是最具侵入性的蚊子之一，其地理分布不断增加。迄今为止，传统的控制方法未能控制AE。白纹伊蚊足够。基于遗传学的新颖策略为有效控制这种蚊子提供了一种有前途的替代方案或帮助。我们在这里描述了 Ae 的分离、表征和用途。白纹伊蚊Actin-4基因驱动伊蚊间接飞行肌肉中的显性致死基因。白纹伊蚊，从而诱导有条件的雌性特有的晚效不飞表型。我们还表明，在这种情况下，来自两种 Ae 的 Actin-4 调控区域。白纹伊蚊和伊蚊。埃及伊蚊可用于为任一物种提供有条件的雌性特有的不飞能力。由于传播疾病的雌性丧失行为能力，雌性不会飞的表型包含遗传性别鉴定机制，适合控制伊蚊。白纹伊蚊使用仅限雄性的释放方法作为综合害虫管理策略的一部分。亚洲虎蚊（白纹伊蚊）是一种高度入侵性物种，在过去四十年中利用人类活动从东南亚传播到非洲、北美、南美和欧洲。除了令人讨厌的叮咬滋扰之外，这种蚊子还对公共健康构成重大威胁，它能够传播登革热，并导致 2007 年印度洋基孔肯雅热爆发。众所周知，使用现有方法很难控制这种蚊子，但涉及释放基因不育雄性的控制技术最近在对抗密切相关的黄热病蚊子埃及伊蚊方面显示出可喜的效果。由不育雄性授精的野生雌性会产生无法存活的后代，因此如果释放足够多的不育雄性，目标种群就会崩溃。雌性蚊子即使不育，也会叮咬并可能获得和传播病原体，因此尽量减少此类雌性蚊子的释放至关重要。在这里，我们描述了用于此类控制计划的亚洲虎蚊基因工程品系的开发：雌性蚊子无法飞行，除非用解毒剂饲养，从而促进只有雄性释放。被释放的雄性的女儿也将不会飞，这在野外是一种致命的表型，从而减少了种群数量并防止了疾病传播。
Aedes albopictus, the Asian tiger mosquito, is a vector of several arboviruses including dengue and chikungunya, and is also a significant nuisance mosquito. It is one of the most invasive of mosquitoes with a relentlessly increasing geographic distribution. Conventional control methods have so far failed to control Ae. albopictus adequately. Novel genetics-based strategies offer a promising alternative or aid towards efficient control of this mosquito. We describe here the isolation, characterisation and use of the Ae. albopictus Actin-4 gene to drive a dominant lethal gene in the indirect flight muscles of Ae. albopictus, thus inducing a conditional female-specific late-acting flightless phenotype. We also show that in this context, the Actin-4 regulatory regions from both Ae. albopictus and Ae. aegypti can be used to provide conditional female-specific flightlessness in either species. With the disease-transmitting females incapacitated, the female flightless phenotype encompasses a genetic sexing mechanism and would be suitable for controlling Ae. albopictus using a male-only release approach as part of an integrated pest management strategy. The Asian Tiger mosquito, Aedes albopictus, is a highly invasive species which took advantage of human activity to spread from South-East Asia to Africa, North and South America, and Europe in the past forty years. Beyond the annoying biting nuisance, this mosquito is also a significant public health threat, capable of transmitting dengue and responsible for an important chikungunya outbreak in the Indian Ocean in 2007. This mosquito is notoriously difficult to control using current methods, but control techniques involving the release of genetically sterile males have recently shown promising results against the closely related yellow fever mosquito, Aedes aegypti. Wild females inseminated by sterile males have non-viable progeny so if enough sterile males are released, the target population crashes. Female mosquitoes, even if sterile, would bite and potentially acquire and transmit pathogens, so it is crucial to minimise the release of such females. Here we describe the development of genetically engineered strains of the Asian Tiger mosquito to use in such control programmes: the females are unable to fly unless reared with an antidote, thus facilitating male-only releases. The daughters of released males will also be flightless, a lethal phenotype in the field, thus reducing the population and preventing disease transmission.
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影响因子：
5.4
作者：
RODEMS, SM;FRIESEN, PD
通讯作者：
FRIESEN, PD
影响因子：
3
作者：
Catteruccia F;Crisanti A;Wimmer EA
通讯作者：
Wimmer EA
影响因子：
5.4
作者：
Phuc HK;Andreasen MH;Burton RS;Vass C;Epton MJ;Pape G;Fu G;Condon KC;Scaife S;Donnelly CA;Coleman PG;White-Cooper H;Alphey L
通讯作者：
Alphey L
影响因子：
46.9
作者：
Harris, Angela F.;Nimmo, Derric;Alphey, Luke
通讯作者：
Alphey, Luke
DOI：
10.1073/pnas.1000251107
发表时间：
2010-03-09
影响因子：
11.1
作者：
Fu, Guoliang;Lees, Rosemary S.;Alphey, Luke
通讯作者：
Alphey, Luke