Multi-color palette of fluorescent proteins for imaging the tumor microenvironment of orthotopic tumorgraft mouse models of clinical pancreatic cancer specimens.
Multi-color palette of fluorescent proteins for imaging the tumor microenvironment of orthotopic tumorgraft mouse models of clinical pancreatic cancer specimens.
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DOI：
10.1002/jcb.24099
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发表时间：
2012-07
影响因子：
4
通讯作者：
Hoffman, Robert M.
中科院分区：
文献类型：
--
作者：
Suetsugu, Atsushi;Katz, Matthew;Fleming, Jason;Truty, Mark;Thomas, Ryan;Moriwaki, Hisataka;Bouvet, Michael;Saji, Shigetoyo;Hoffman, Robert M.
胰腺癌患者的肿瘤标本最初是在手术后立即在SCID-NOD小鼠皮下建立的。然后从SCID-NOD小鼠中获取患者肿瘤，并在普遍表达RFP的转基因裸鼠中原位传代。原发患者肿瘤获得了表达RFP的间质。表达RFP的间质包括肿瘤相关成纤维细胞(CAF)和肿瘤相关巨噬细胞(TAM)。进一步传代到普遍表达GFP的转基因裸鼠身上会导致肿瘤和转移，这些裸鼠除了获得RFP基质外，还获得了GFP基质，包括CAF、TAM和血管。RFP间质在生长于GFP小鼠体内的肿瘤中持续存在。进一步传代到普遍表达CFP的转基因裸鼠，肿瘤和转移除了获得RFP和GFP间质外，还获得了CFP间质，包括表达RFP和GFP的CAF和TAM和血管。该模型可用于成像患者胰腺肿瘤的原发和转移进展，以直观地靶向间质和癌细胞，并实现个体化治疗。
Pancreatic-cancer-patient tumor specimens were initially established subcutaneously in SCID-NOD mice immediately after surgery. The patient tumors were then harvested from SCID-NOD mice and passaged orthotopically in transgenic nude mice ubiquitously expressing RFP. The primary patient tumors acquired RFP-expressing stroma. The RFP-expressing stroma included cancer-associated fibroblasts (CAFs) and tumor-associated macrophages (TAMs). Further passage to transgenic nude mice ubiquitously expressing GFP resulted in tumors and metastasis that acquired GFP stroma in addition to their RFP stroma, including CAFs and TAMs and blood vessels. The RFP stroma persisted in the tumors growing in the GFP mouse. Further passage to transgenic nude mice ubiquitously expressing CFP resulted in tumors and metastasis acquiring CFP stroma in addition to persisting RFP and GFP stroma including RFP- and GFP-expressing CAFs and TAMs and blood vessels. This model can be used to image primary and metastatic progression of patient pancreatic tumors to visually target stroma as well as cancer cells and individualize therapy.
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影响因子：
3.6
作者：
Embuscado, EE;Laheru, D;Iacobuzio-Donahue, CA
通讯作者：
Iacobuzio-Donahue, CA
DOI：
10.1073/pnas.89.12.5645
发表时间：
1992-06-15
影响因子：
11.1
作者：
FU, XY;GUADAGNI, F;HOFFMAN, RM
通讯作者：
HOFFMAN, RM
影响因子：
8.8
作者：
NOWAK, K;PECKHAM, MJ;STEEL, GG
通讯作者：
STEEL, GG
影响因子：
11.2
作者：
Duda, DG;Fukumura, D;Jain, RK
通讯作者：
Jain, RK
DOI：
10.1073/pnas.88.20.9345
发表时间：
1991-10-01
影响因子：
11.1
作者：
FU, XY;BESTERMAN, JM;HOFFMAN, RM
通讯作者：
HOFFMAN, RM