Quantitative measurement of regional cerebral blood flow and oxygen metabolism in a rat model of cerebral hypoperfusion
Quantitative measurement of regional cerebral blood flow and oxygen metabolism in a rat model of cerebral hypoperfusion
复制标题
脑低灌注大鼠模型局部脑血流及氧代谢的定量测定
DOI：
10.1016/j.brainres.2019.05.032
复制
发表时间：
2019
期刊：
影响因子：
2.9
通讯作者：
Hatazawa Jun
中科院分区：
文献类型：
--
作者：
Kato Hiroki;Kanai Yasukazu;Watabe Tadashi;Ikeda Hayato;Horitsugi Genki;Hatazawa Jun
本研究旨在探讨双侧颈总动脉闭塞（BCAO）模型大鼠脑氧代谢率（cmoro2）与脑血流量（CBF）的区域变化。对10周龄雄性Wistar大鼠进行双侧颈总动脉结扎（或对照动物的假手术）。在麻醉动物的亚急性期（手术后1 周）和慢性期（手术后6 周），使用小动物PET系统和我们之前研究开发的O-15气体稳态吸入法动脉采血，获得O-15 PET图像。kl<s:1> ver- barrera法进行组织病理学染色，胶质原纤维酸性蛋白免疫细胞化学染色。采用y型迷宫测试认知功能。BCAO大鼠亚急性期脑血流显著降低，脑大区域氧萃取率显著升高，慢性期脑血流恢复。BCAO大鼠动脉闭塞亚急性期及以后cro_2稳定下降，主要发生在大脑前动脉区域。慢性期胼胝体萎缩，胼胝体萎缩。BCAO中星形胶质细胞的活性在两期均显著。慢性期BCAO的工作记忆受损。基于稳态法定量O-15 PET观察大鼠动脉闭塞亚急性期和慢性期脑灌注和氧代谢的局部变化。
This study was aimed at evaluating the regional changes in the cerebral metabolic rate of oxygen (CMRO2) in relation to the cerebral blood flow (CBF) in the bilateral common carotid artery occlusion (BCAO) rat model.Ligation of the bilateral common carotid arteries (or a sham operation in control animals) was performed in 10-week-old male Wistar rats. O-15 PET images were acquired in the subacute phase (1 week after the surgery) and chronic phase (6 weeks after the surgery) with the animals under anesthesia, using a small-animal PET system and the O-15 gas steady-state inhalation method with arterial blood sampling developed in our previous study. Histopathological staining by Klüver-Barrera method and immunocytochemistry staining by glial fibrillary acidic protein were performed. Cognitive function was tested by using the apparatus of Y-maze.Significantly lower CBF and higher oxygen extraction fraction were observed in broad areas of the cerebrum in the subacute phase in the BCAO rats, with recovery in the chronic phase. A stable decrease of the CMRO2in the subacute phase of arterial occlusion and later was observed in the BCAO rat model, mainly in the anterior cerebral artery territory. Atrophy and rarefaction of corpus callosum were found in the BCAO in the chronic phase. Activity of astrocytes in the BCAO was prominent in the both phases. Working memory was impaired in the BCAO in the chronic phase.Regional changes in cerebral perfusion and oxygen metabolism in the subacute and chronic phases of arterial occlusion were clarified in a rat model of BCAO by quantitative O-15 PET based on the steady-state method.
登录
查看更多内容
一种测定大鼠大脑血流量和耗氧量的方法。
DOI：
--
发表时间：
1976
期刊：
Acta Physiologica Scandinavica
影响因子：
--
作者：
B. Nilsson;B. Siesjö
通讯作者：
B. Siesjö
影响因子：
8.3
作者：
A. Matsumoto;Y. Utsunomiya;K. Kogure;P. Scheinberg;O. Reinmuth
通讯作者：
O. Reinmuth
影响因子：
8.3
作者：
COYLE, P;PANZENBECK, MJ
通讯作者：
PANZENBECK, MJ
影响因子：
2.6
作者：
JONES, T;CHESLER, DA;TERPOGOSSIAN, MM
通讯作者：
TERPOGOSSIAN, MM
测量大脑局部氧提取率的稳态技术中血管内 Oxygen-15 存在的校正： 1. 方法说明
影响因子：
6.3
作者：
A. Lammertsma;T. Jones
通讯作者：
T. Jones