Dynamic alterations in monocyte numbers, subset frequencies and activation markers in acute and convalescent COVID-19 individuals.
Dynamic alterations in monocyte numbers, subset frequencies and activation markers in acute and convalescent COVID-19 individuals.
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DOI：
10.1038/s41598-021-99705-y
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发表时间：
2021-10-12
影响因子：
4.6
通讯作者：
Babu S
中科院分区：
文献类型：
--
作者：
Rajamanickam A;Kumar NP;Pandiarajan AN;Selvaraj N;Munisankar S;Renji RM;Venkatramani V;Murhekar M;Thangaraj JWV;Kumar MS;Kumar CPG;Bhatnagar T;Ponnaiah M;Sabarinathan R;Saravanakumar V;Babu S
单核细胞被认为在COVID-19的宿主防御和发病机制中发挥重要作用。然而，尚未对COVID-19急性期和恢复期的单核细胞数量和功能进行全面的纵向检查。我们使用流式细胞术和ELISA检测了7组COVID-19个体的单核细胞绝对计数、单核细胞亚群的频率、单核细胞激活标志物的血浆水平，根据RT-PCR确认SARS-CoV2感染的天数进行分类。我们的数据显示，总单核细胞的绝对计数和中间和非经典单核细胞的频率从15-30天增加到61-90天，之后趋于平稳。相比之下，经典单核细胞的频率从第15-30天到第121-150天下降。血浆中sCD14、CRP、sCD163和组织因子(sTF) -的水平从15-30天下降至151-180天。与轻症患者相比，重症患者单核细胞计数水平较高，经典单核细胞频率较高，中间和非经典单核细胞频率较低，血浆sCD14、CRP、sCD163和sTF水平升高。因此，我们的研究为急性和恢复期COVID-19个体的单核细胞计数、亚群频率和激活状态的动态变化提供了证据。
Monocytes are thought to play an important role in host defence and pathogenesis of COVID-19. However, a comprehensive examination of monocyte numbers and function has not been performed longitudinally in acute and convalescent COVID-19. We examined the absolute counts of monocytes, the frequency of monocyte subsets, the plasma levels of monocyte activation markers using flowcytometry and ELISA in seven groups of COVID-19 individuals, classified based on days since RT-PCR confirmation of SARS-CoV2 infection. Our data shows that the absolute counts of total monocytes and the frequencies of intermediate and non-classical monocytes increases from Days 15–30 to Days 61–90 and plateau thereafter. In contrast, the frequency of classical monocytes decreases from Days 15–30 till Days 121–150. The plasma levels of sCD14, CRP, sCD163 and sTissue Factor (sTF)—all decrease from Days 15–30 till Days 151–180. COVID-19 patients with severe disease exhibit higher levels of monocyte counts and higher frequencies of classical monocytes and lower frequencies of intermediate and non-classical monocytes and elevated plasma levels of sCD14, CRP, sCD163 and sTF in comparison with mild disease. Thus, our study provides evidence of dynamic alterations in monocyte counts, subset frequencies and activation status in acute and convalescent COVID-19 individuals.
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DOI：
10.1186/s12941-020-00362-2
发表时间：
2020-05-15
影响因子：
5.7
作者：
Chen, Wei;Zheng, Kenneth I.;Qiao, Zengpei
通讯作者：
Qiao, Zengpei
影响因子：
2.9
作者：
Hodges G;Pallisgaard J;Schjerning Olsen AM;McGettigan P;Andersen M;Krogager M;Kragholm K;Køber L;Gislason GH;Torp-Pedersen C;Bang CN
通讯作者：
Bang CN
影响因子：
3.6
作者：
Prame Kumar K;Nicholls AJ;Wong CHY
通讯作者：
Wong CHY
DOI：
10.1038/nri3070
发表时间：
2011-10-10
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1097/qai.0b013e3182910121
发表时间：
2013-07-01
期刊：
Journal of acquired immune deficiency syndromes (1999)
影响因子：
--
作者：
Baker JV;Huppler Hullsiek K;Bradford RL;Prosser R;Tracy RP;Key NS
通讯作者：
Key NS