High-Resolution Detection of Translocation of Oral Bacteria to the Gut.
High-Resolution Detection of Translocation of Oral Bacteria to the Gut.
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DOI：
10.1177/00220345231160747
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发表时间：
2023-07
影响因子：
7.6
通讯作者：
中科院分区：
文献类型：
--
作者：
口腔微生物在肠道中的异位富集是肠道微生物平衡的显著改变。这些微生物可能通过唾液和食物从口腔中传播;然而，口腔肠道微生物传播的证据不足，需要进一步调查。在这项观察性研究中，我们检查了从社区居民中收集的144对唾液和粪便样本，以验证口腔-肠道微生物的联系，并确定肠道内口腔微生物丰度增加的相关影响因素。使用全长16 S核糖体RNA基因的PacBio单分子长读段测序和扩增子序列变体（ASV）分析确定每份样品的细菌组成。虽然唾液和肠道菌群的细菌组成明显不同，但在72.9%的受试者中，唾液和肠道菌群之间至少共享1个ASV。共享ASV占每例受试者肠道微生物群的0.0%至63.1%（中位数0.14%），通常包括丰富的唾液链球菌和副血链球菌。它们在肠道中的总相对丰度在老年受试者或有牙菌斑积累的受试者中显着较高。具有≥5%共享ASV的肠道微生物群显示出较高丰度的链球菌属、乳杆菌属和克雷伯氏菌属，以及较低丰度的粪杆菌属、布劳特氏菌属、巨单胞菌属和副杆菌属。我们的研究提供了社区居民口腔细菌移位到肠道的证据，并表明衰老和牙菌斑积累有助于肠道中口腔微生物丰度的增加，这可能与肠道菌群的组成变化有关。
Ectopic enrichment of oral microbes in the gut is a notable alteration in gut microbial balance. These microbes are likely delivered from the oral cavity with saliva and food; however, evidence of oral–gut microbial transmission is insufficient and needs further investigation. In this observational study, we examined 144 pairs of saliva and stool samples collected from community-dwelling adults to verify the oral–gut microbial link and identify the relevant influencing factors on the increased abundance of oral microbes within the gut. The bacterial composition of each sample was determined using PacBio single-molecule long-read sequencing of the full-length 16S ribosomal RNA gene and amplicon sequence variant (ASV) analysis. Although the bacterial compositions of salivary and gut microbiota were distinctly different, at least 1 ASV was shared between salivary and gut microbiota in 72.9% of subjects. Shared ASVs accounted for 0.0% to 63.1% (median 0.14%) of the gut microbiota in each subject and frequently included abundant Streptococcus salivarius and Streptococcus parasanguinis. Their total relative abundance in the gut was significantly higher in older subjects or those with dental plaque accumulation. The gut microbiota with ≥5% of shared ASVs displayed a higher abundance of Streptococcus, Lactobacillus, and Klebsiella and a lower abundance of Faecalibacterium, Blautia, Megamonas, and Parabacteroides. Our study presents evidence for the translocation of oral bacteria to the gut in community-dwelling adults and suggests that aging and dental plaque accumulation contribute to an increased abundance of oral microbes in the gut, which might be relevant to the compositional shift in the gut commensals.
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DOI：
10.1126/science.aan4526
发表时间：
2017-10-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Atarashi K;Suda W;Luo C;Kawaguchi T;Motoo I;Narushima S;Kiguchi Y;Yasuma K;Watanabe E;Tanoue T;Thaiss CA;Sato M;Toyooka K;Said HS;Yamagami H;Rice SA;Gevers D;Johnson RC;Segre JA;Chen K;Kolls JK;Elinav E;Morita H;Xavier RJ;Hattori M;Honda K
通讯作者：
Honda K
影响因子：
6.4
作者：
Kageyama S;Furuta M;Takeshita T;Ma J;Asakawa M;Yamashita Y
通讯作者：
Yamashita Y
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
12.3
作者：
Segata N;Haake SK;Mannon P;Lemon KP;Waldron L;Gevers D;Huttenhower C;Izard J
通讯作者：
Izard J
影响因子：
6.3
作者：
Tanaka, Yukari;Yamashita, Riu;Kawashima, Junko;Mori, Hiroshi;Kurokawa, Ken;Fukuda, Shinji;Gotoh, Yasuhiro;Nakamura, Keiji;Hayashi, Tetsuya;Kasahara, Yoshiyuki;Sato, Yukuto;Fukudo, Shin
通讯作者：
Fukudo, Shin