A dynamic mouse peptidome landscape reveals probiotic modulation of the gut-brain axis
A dynamic mouse peptidome landscape reveals probiotic modulation of the gut-brain axis
复制标题
动态小鼠肽组景观揭示了益生菌对肠脑轴的调节
DOI：
10.1126/scisignal.abb0443
复制
发表时间：
2020-07
影响因子：
7.3
通讯作者：
Jia Chenxi
中科院分区：
文献类型：
--
作者：
Zhang Pei;Wu Xiaoli;Liang Shan;Shao Xianfeng;Wang Qianqian;Chen Ruibing;Zhu Weimin;Shao Chen;Jin Feng;Jia Chenxi
益生菌治疗改变了大脑中神经肽的丰度。除了在调节宿主代谢和免疫方面发挥关键作用外，肠道微生物组还在肠-脑轴中发挥重要作用，肠-脑轴是中枢和肠神经系统之间的双向交流系统。Zhang等人分析了喂食两种不同益生菌的小鼠的脑肽组和肠道微生物组。益生菌治疗改变了许多神经肽的丰度，其方式与肠道微生物组组成的变化相关，因脑区而异，并取决于益生菌种类，益生菌是活的还是热灭活的，以及治疗的持续时间。这些组学数据集是未来研究肠道微生物群与神经肽控制的认知，行为和生理过程之间的机制联系的基础。某些益生菌通过调节肠-脑轴对中枢神经系统的功能具有有益作用。在这里，我们描述了跨多个大脑区域的肽组的动态景观，通过在不同时间口服不同的益生菌种类来调节。脑肽组的时空和菌株特异性变化与肠道微生物组的组成相关。海马体对益生菌治疗表现出最敏感的反应。热灭活益生菌的施用改变了海马肽组，但没有实质性改变肠道微生物组。我们开发了一种文献挖掘算法，将益生菌改变的神经肽与潜在的功能作用联系起来。我们通过监测下丘脑-垂体-肾上腺轴验证了促肾上腺皮质激素释放激素的益生菌调节作用，益生菌治疗可减弱产前应激诱导的过度活跃。我们的研究结果为益生菌调节脑肽组提供了证据，并为进一步研究肠-脑轴和益生菌疗法提供了资源。
Probiotic treatment alters the abundance of neuropeptides in the brain. The brain on probiotics Besides being a key player in the regulation of host metabolism and immunity, the gut microbiome also plays an important role in the gut-brain axis, a system of bidirectional communication between the central and enteric nervous systems. Zhang et al. analyzed the brain peptidomes and gut microbiomes of mice fed two different species of probiotics. Probiotic treatment altered the abundances of many neuropeptides in a manner that correlated with changes in the composition of the gut microbiome, varied by brain region, and depended on the probiotic species, whether the probiotic was live or heat-killed, and the duration of treatment. These omics datasets are a foundation for future studies of the mechanistic links between the gut microbiota and cognitive, behavioral, and physiological processes controlled by neuropeptides. Certain probiotics have beneficial effects on the function of the central nervous system through modulation of the gut-brain axis. Here, we describe a dynamic landscape of the peptidome across multiple brain regions, modulated by oral administration of different probiotic species over various times. The spatiotemporal and strain-specific changes of the brain peptidome correlated with the composition of the gut microbiome. The hippocampus exhibited the most sensitive response to probiotic treatment. The administration of heat-killed probiotics altered the hippocampus peptidome but did not substantially change the gut microbiome. We developed a literature-mining algorithm to link the neuropeptides altered by probiotics with potential functional roles. We validated the probiotic-regulated role of corticotropin-releasing hormone by monitoring the hypothalamic-pituitary-adrenal axis, the prenatal stress–induced hyperactivity of which was attenuated by probiotics treatment. Our findings provide evidence for modulation of the brain peptidome by probiotics and provide a resource for further studies of the gut-brain axis and probiotic therapies.
登录
查看更多内容
影响因子：
--
作者：
Karen A Neufeld;N. Kang;J. Bienenstock;J. Foster
通讯作者：
Karen A Neufeld;N. Kang;J. Bienenstock;J. Foster
影响因子：
4.6
作者：
Jafari Z;Mehla J;Kolb BE;Mohajerani MH
通讯作者：
Mohajerani MH
影响因子：
4.6
作者：
Harach T;Marungruang N;Duthilleul N;Cheatham V;Mc Coy KD;Frisoni G;Neher JJ;Fåk F;Jucker M;Lasser T;Bolmont T
通讯作者：
Bolmont T
影响因子：
5.6
作者：
Liang S;Wu X;Hu X;Wang T;Jin F
通讯作者：
Jin F
影响因子：
14.9
作者：
Ma J;Chen T;Wu S;Yang C;Bai M;Shu K;Li K;Zhang G;Jin Z;He F;Hermjakob H;Zhu Y
通讯作者：
Zhu Y