Application of induced pluripotent stem (iPS) cells in periodontal tissue regeneration.
Application of induced pluripotent stem (iPS) cells in periodontal tissue regeneration.
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DOI：
10.1002/jcp.22316
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发表时间：
2011-01
影响因子：
5.6
通讯作者：
Chen, Jake
中科院分区：
文献类型：
--
作者：
Duan, Xuejing;Tu, Qisheng;Zhang, Jin;Ye, Jinhai;Sommer, Cesar;Mostoslavsky, Gustavo;Kaplan, David;Yang, Pishan;Chen, Jake
组织工程为牙周组织再生提供了新的模式，合适的干细胞和有效的细胞因子在其中是非常重要的。本研究的目的是，第一次，调查牙周组织再生的能力和优势，使用诱导多能干细胞（iPS）细胞和釉基质衍生物（EMD）。本研究首先在体外检测EMD凝胶对iPS细胞的影响，然后采用组织工程技术修复牙周缺损，分为3组：单纯丝素支架组、丝素支架+ EMD组和丝素支架+ EMD + iPS细胞组。EMD显著促进Runx2 mRNA的表达，但抑制OC mRNA的表达和矿化结节的形成。移植的iPS细胞表现出较高的表达水平的OC，Osx和Runx2基因，术后12和24天。术后24 d，iPS细胞组牙槽骨、牙骨质及牙周膜组织学观察显示，新牙结果表明，EMD有助于间充质祖细胞向成骨谱系中的早期细胞的定向作用。iPS细胞与EMD联合应用，可促进新牙骨质、牙槽骨和正常牙周膜的形成，为牙周组织工程提供了一种有价值的工具。
Tissue engineering provides a new paradigm for periodontal tissue regeneration in which proper stem cells and effective cellular factors are very important. The objective of this study was, for the first time, to investigate the capabilities and advantages of periodontal tissue regeneration using induced pluripotent stem (iPS) cells and enamel matrix derivatives (EMD). In this study the effect of EMD gel on iPS cells in vitro was first determined, and then tissue engineering technique was performed to repair periodontal defects in three groups: silk scaffold only; silk scaffold + EMD; and silk scaffold + EMD + iPS cells. EMD greatly enhanced the mRNA expression of Runx2 but inhibited the mRNA expression of OC and mineralization nodule formation in vitro. Transplantation of iPS cells showed higher expression levels of OC, Osx, and Runx2 genes, both 12 and 24 days postsurgery. At 24 days postsurgery in the iPS cell group, histological analysis showed much more new alveolar bone and cementum formation with regenerated periodontal ligament between them. The results showed the commitment role that EMD contributes in mesenchymal progenitors to early cells in the osteogenic lineage. iPS cells combined with EMD provide a valuable tool for periodontal tissue engineering, by promoting the formation of new cementum, alveolar bone, and normal periodontal ligament.
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影响因子：
64.5
作者：
Park IH;Arora N;Huo H;Maherali N;Ahfeldt T;Shimamura A;Lensch MW;Cowan C;Hochedlinger K;Daley GQ
通讯作者：
Daley GQ
影响因子：
3.6
作者：
Kemoun, Philippe;Laurencin-Dalicieux, Sara;Salles, Jean-Pierre
通讯作者：
Salles, Jean-Pierre
影响因子：
64.5
作者：
Nakashima, K;Zhou, X;de Crombrugghe, B
通讯作者：
de Crombrugghe, B
影响因子：
14
作者：
Jiang, Xinquan;Zhao, Jun;Wang, Shaoyi;Sun, Xiaojuan;Zhang, Xiuli;Chen, Jake;Kaplan, David L.;Zhang, Zhiyuan
通讯作者：
Zhang, Zhiyuan
影响因子：
3.5
作者：
Nagatomo, K;Komaki, M;Ishikawa, I
通讯作者：
Ishikawa, I