Involvement of PhoP/PhoQ two-component system in biofilm formation in Cronobacter sakazakii
Involvement of PhoP/PhoQ two-component system in biofilm formation in Cronobacter sakazakii
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PhoP/PhoQ 双组分系统参与坂崎克罗诺杆菌生物膜形成
DOI：
10.1016/j.foodcont.2021.108621
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发表时间：
2021-10
期刊：
影响因子：
6
通讯作者：
Xia Xiaodong
中科院分区：
文献类型：
--
作者：
Ma Yan;Zhang Yingying;Shan Zhongguo;Wang Xin;Xia Xiaodong
研究了PhoP/PhoQ双组分体系对坂崎克罗伊氏菌BAA-894挂膜的影响。结晶紫染色法和XTT法显示，Δ phoPQC菌株生物膜生物量和生物膜细胞活力明显降低，而互补菌株ΔphoPQC则逆转了上述缺陷。此外，phoPQ基因的缺失减弱了BAA-894菌株在玻璃和硅片上形成生物膜的能力，并影响了在不锈钢板上形成的生物膜中胞外聚合物（EPS）的含量。此外，qRT-PCR结果表明，C.坂崎在调节与鞭毛和纤维素生物合成相关的几个基因的表达中发挥作用，这两个基因都参与生物膜的形成。这些结果表明，PhoP/PhoQ系统在生物膜形成中起着重要作用。sakazakii和该系统可以被认为是开发用于控制生物膜的新的替代策略的潜在目标。
The aim of this study was to investigated the effect of PhoP/PhoQ two component system on the biofilm formation ofCronobacter sakazakiiBAA-894. Crystal violet staining assay and XTT assay showed that the biofilm biomass and the viability of biofilm cells were significantly reduced in ΔphoPQstrain, while these defects were reversed in the complementation strain ΔphoPQC. Moreover, thephoPQgene deletion weakened the ability of BAA-894 strain to form biofilms on glass and silicon wafers, and affected the content of extracellular polymeric substance (EPS) in biofilms formed on stainless-steel plates. In addition, qRT-PCR results showed that the PhoP/PhoQ system ofC. sakazakiiplayed roles in regulating the expression of several genes associated with flagella and cellulose biosynthesis, which are both involved in biofilm formation. These finding suggest that the PhoP/PhoQ system plays important roles in biofilm formation inC. sakazakiiand the system could be considered as a potential target for developing novel alternative strategies for controlling biofilms.
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影响因子：
3.6
作者：
Zwir I;Latifi T;Perez JC;Huang H;Groisman EA
通讯作者：
Groisman EA
影响因子：
3.8
作者：
Daoyi Xi;Shuang Yang;Qian Liu;Yujia Li;Yuehua Li;Junxiang Yan;Xiaochen Wang;Kexin Ning;Boyang Cao
通讯作者：
Daoyi Xi;Shuang Yang;Qian Liu;Yujia Li;Yuehua Li;Junxiang Yan;Xiaochen Wang;Kexin Ning;Boyang Cao
影响因子：
5.2
作者：
Li W;Li Y;Liu Y;Shi X;Jiang M;Lin Y;Qiu Y;Zhang Q;Chen Q;Zhou L;Sun Q;Hu Q
通讯作者：
Hu Q
影响因子：
5.2
作者：
P. Cal-Sabater;I. Caro;M. J. Castro;María-José Cao;J. Mateo;E. J. Quinto
通讯作者：
P. Cal-Sabater;I. Caro;M. J. Castro;María-José Cao;J. Mateo;E. J. Quinto
DOI：
10.1016/j.jmii.2019.06.003
发表时间：
2020-08
期刊：
Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi
影响因子：
--
作者：
M. Tsai;Yi-Hua Liang;Chyi-Liang Chen;C. Chiu
通讯作者：
M. Tsai;Yi-Hua Liang;Chyi-Liang Chen;C. Chiu