Population decline of an endangered unionid, Pronodularia japanensis, in streams is revealed by eDNA and conventional monitoring approaches
Population decline of an endangered unionid, Pronodularia japanensis, in streams is revealed by eDNA and conventional monitoring approaches
复制标题
eDNA 和传统监测方法揭示了溪流中濒危尤氏原虫 (Pronodularia japanensis) 的数量下降
DOI：
10.1007/s10750-022-04852-6
复制
发表时间：
2022
期刊：
影响因子：
2.6
通讯作者：
Yamashita Naoki
中科院分区：
文献类型：
--
作者：
Hata Hiroki;Ogasawara Kota;Yamashita Naoki
环境DNA（eDNA）可用于检测水样中是否存在水生生物，特别是稀有和隐蔽物种。在日本西南部的松山平原，两种淡水蚌类--日本前节蚌（Pronodularia japanensis）和道格拉斯节蚌（Nodularia douglasiae）在过去30年中迅速减少，目前正面临灭绝的威胁。我们设计了一个种特异性的cDNA标记，靶向的COI区域的P。利用该cDNA标记的定量PCR技术和常规调查方法，对该种在松山平原的Kunichi水系的分布进行了调查。结果表明，P. 2013-2014年和2020-2021年期间，日本人口没有变化，但其密度下降了99%。eDNA of P.在人工采集该物种的地点，100%成功地检测到了japanensis。此外，在P.未收集到日本脑炎，但预计会发生。本研究建立了一个针对P. japanensis的cDNA序列，该cDNA标记可有效地用于该种的分布调查。使用这种eDNA标记，对剩余种群的广泛调查和对它们的监测应该改善保护措施。
Environmental DNA (eDNA) is useful to detect the presence of aquatic organisms from water samples, especially for rare and cryptic species. Two freshwater unionid mussels¸Pronodularia japanensisandNodularia douglasiae, have rapidly declined over the last three decades and are now threatened with extinction on the Matsuyama Plain, south-western Japan. We designed a species-specific eDNA marker targeting the COI region ofP. japanensis.The distribution of this species in the Kunichi River system on the Matsuyama Plain was investigated using both quantitative PCR with this eDNA marker and conventional surveying. We show that the distribution area ofP. japanensisdid not change between 2013–2014 and 2020–2021, but its density decreased by 99%. eDNA ofP. japanensiswas detected, with 100% success, from sites where this species was collected by hand. Furthermore, eDNA was detected at nine sites whereP. japanensiswas not collected but was expected to occur. This study has established a species-specific eDNA marker targeting the COI region ofP. japanensis, and this eDNA marker has been validated as effective for surveying the distribution of this species. Using this eDNA marker, extensive investigation of remaining populations and the monitoring of them should improve conservation practices.
登录
查看更多内容
DOI：
10.1002/edn3.171
发表时间：
2020-12
期刊：
--
影响因子：
--
作者：
B. Thalinger;D. Kirschner;Yannick Pütz;Christian Moritz;Richard Schwarzenberger;J. Wanzenböck;M. Traugott
通讯作者：
B. Thalinger;D. Kirschner;Yannick Pütz;Christian Moritz;Richard Schwarzenberger;J. Wanzenböck;M. Traugott
贻贝环境 DNA 的河流分布反映了密度、运输和清除过程之间的平衡
影响因子：
2.7
作者：
Arial J. Shogren;J. Tank;S. Egan;D. Bolster;T. Riis
通讯作者：
T. Riis
苦鳅（Rhodeus Amarus Bloch）繁殖行为的行为学分析
DOI：
10.1163/036551661x00052
发表时间：
1962
期刊：
--
影响因子：
--
作者：
P. R. Wiepkema
通讯作者：
P. R. Wiepkema
影响因子：
10.7
作者：
Curole, JP;Kocher, TD
通讯作者：
Kocher, TD
淡水珍珠贝 (Margaritifera margaritifera) eDNA 浓度的下游运输和季节变化
影响因子：
--
作者：
S. Wacker;F. Fossøy;B. M. Larsen;Hege Brandsegg;R. Sivertsgård;S. Karlsson
通讯作者：
S. Karlsson