Differential effect of tantalum nanoparticles versus tantalum micron particles on immune regulation.
Differential effect of tantalum nanoparticles versus tantalum micron particles on immune regulation.
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DOI：
10.1016/j.mtbio.2022.100340
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发表时间：
2022-12
影响因子：
8.2
通讯作者：
Hu, Yihe
中科院分区：
文献类型：
--
作者：
Sun, Yan;Liu, Tuozhou;Hu, Hongkun;Xiong, Zixuan;Zhang, Kai;He, Xi;Liu, Wenbin;Lei, Pengfei;Hu, Yihe
巨噬细胞产生的炎性微环境已被证明是骨再生的关键。纳米钽和微米颗粒均已应用于骨组织工程中，并取得了良好的效果，但其对免疫微环境的影响尚不清楚。在此，我们探讨了纳米和微米级的钽颗粒在体内和体外对巨噬细胞天然免疫功能的不同影响。将RAW 264.7细胞与纳米和微米级钽颗粒在炎症条件下共培养，以评估其对巨噬细胞形态和行为的影响。采用气囊模型评价材料对体内巨噬细胞极化的影响。与微米级钽微粒(TAMPS)相比，与纳米级钽微粒(TaNPs)共培养时，巨噬细胞的形态更接近M2表型。同时，还能降低白介素1β、肿瘤坏死因子β、诱导型一氧化氮合酶的基因表达，上调转化生长因子β1、白介素10的表达。此外，气囊模型显示在TaNPs干预下有更多的M2巨噬细胞浸润。结果表明，在炎症微环境下，TaNPs能显著增加M2巨噬细胞的极化，降低巨噬细胞对M1表型的极化，表现出更好的免疫调节特性。首次比较了钽颗粒对巨噬细胞的免疫调节作用。在炎症环境中，纳米钽颗粒可以抑制巨噬细胞向M1表型的极化，促进巨噬细胞向M2表型的转变。纳米钽可以通过降低细胞内ROS来影响巨噬细胞的极化。
The inflammatory microenvironment created by macrophages has been proven critical for bone regeneration. Both tantalum nanoparticles and micron particles have been applied to bone tissue engineering and have achieved good efficacy, but their effects on immune microenvironment remain unclear. Herein, we explored the different effects between nano- and micro-tantalum particles on the innate immunity of macrophages in vitro and in vivo. RAW 264.7 cells were co-cultured with nano- and micro-tantalum particles under inflammatory conditions to evaluate the effects on the morphology and behavior of macrophages. Air pouch model was used to evaluate the material-induced macrophage polarization in vivo. Compared to the tantalum micron particles (TaMPs), the morphology of macrophages was more similar to the M2 phenotype in co-culture with tantalum nanoparticles (TaNPs). At the same time, the TaNPs could also decrease the gene expression of interleukin-1β (IL-1β), tumor necrosis factor-α(TNF-α), inducible nitric oxide synthase (iNOS), and increase the expression of transforming growth factor-β1 (TGF-β1) and interleukin-10 (IL-10). Furthermore, the air pouch model showed more M2 macrophage infiltration under the intervention of TaNPs. Our findings demonstrated that TaNPs could significantly increase the polarization of M2 macrophages and decrease the macrophage polarization to the M1 phenotype under the inflammatory microenvironment, showing better immunomodulatory properties. The first comparison of the immunomodulatory effects of tantalum particles on macrophages. Tantalum nanoparticles can inhibit the polarization of macrophages to M1 phenotype and promote them to M2 phenotype in an inflammatory environment. Tantalum nanoparticles can affect the polarization of macrophages by reducing intracellular ROS.
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