Monocolonization of germ-free mice with Bacteroides fragilis protects against dextran sulfate sodium-induced acute colitis.
Monocolonization of germ-free mice with Bacteroides fragilis protects against dextran sulfate sodium-induced acute colitis.
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DOI：
10.1155/2014/675786
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发表时间：
2014
影响因子：
--
通讯作者：
Chuang HL
中科院分区：
文献类型：
--
作者：
Chiu CC;Ching YH;Wang YC;Liu JY;Li YP;Huang YT;Chuang HL
溃疡性结肠炎是由遗传因素和环境因素相互作用引起的结肠炎性疾病。以往的研究表明肠道菌群可能参与了结肠炎的发生。脆弱类杆菌是一种革兰氏阴性厌氧菌，属于结肠共生菌。目的：探讨补肾活血方对葡聚糖硫酸钠(DSS)诱导的无菌小鼠结肠炎模型的保护作用。GC57BL/6JNarl小鼠接种BF 28d后，用DSS诱导小鼠急性结肠炎。BF定植后动物存活率显著提高40%，结肠长度减少较少，结肠粘膜炎性细胞(巨噬细胞和中性粒细胞)的浸润减少。此外，补肾活血方还可通过细胞因子IL-17的多态作用增强IL-10等抗炎相关细胞因子的基因表达，通过诱导型一氧化氮合酶抑制促炎相关的肿瘤坏死因子α的表达。BF-DSS小鼠的髓过氧化物酶活性也降低。综上所述，BF定植通过抑制炎症相关分子的活性和诱导抗炎细胞因子的产生而显著改善DSS诱导的结肠炎。BF可能在维持肠道免疫系统动态平衡和调节炎症反应中发挥重要作用。
Ulcerative colitis is inflammatory conditions of the colon caused by interplay of genetic and environmental factors. Previous studies indicated that the gut microflora may be involved in the colonic inflammation. Bacteroides fragilis (BF) is a Gram-negative anaerobe belonging to the colonic symbiotic. We aimed to investigate the protective role of BF in a colitis model induced in germ-free (GF) mice by dextran sulfate sodium (DSS). GF C57BL/6JNarl mice were colonized with BF for 28 days before acute colitis was induced by DSS. BF colonization significantly increased animal survival by 40%, with less reduction in colon length, and decreased infiltration of inflammatory cells (macrophages and neutrophils) in colon mucosa following challenge with DSS. In addition, BF could enhance the mRNA expression of anti-inflammatory-related cytokine such as interleukin 10 (IL-10) with polymorphism cytokine IL-17 and diminish that of proinflammatory-related tumor necrosis factor α with inducible nitric oxide synthase in the ulcerated colon. Myeloperoxidase activity was also decreased in BF-DSS mice. Taking these together, the BF colonization significantly ameliorated DSS-induced colitis by suppressing the activity of inflammatory-related molecules and inducing the production of anti-inflammatory cytokines. BF may play an important role in maintaining intestinal immune system homeostasis and regulate inflammatory responses.
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DOI：
10.1016/j.ijfoodmicro.2006.09.018
发表时间：
2007-03-20
影响因子：
5.4
作者：
Geier, Mark S.;Butler, Ross N.;Howarth, Gordon S.
通讯作者：
Howarth, Gordon S.
影响因子：
4.3
作者：
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通讯作者：
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影响因子：
30.5
作者：
通讯作者：
--
影响因子：
4
作者：
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通讯作者：
MacDonald, Thomas T.
影响因子：
64.5
作者：
Mazmanian, SK;Liu, CH;Kasper, DL
通讯作者：
Kasper, DL