Altered motility of Caulobacter Crescentus in viscous and viscoelastic media.
Altered motility of Caulobacter Crescentus in viscous and viscoelastic media.
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DOI：
10.1186/s12866-014-0322-3
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发表时间：
2014-12-24
期刊：
影响因子：
4.2
通讯作者：
Tang JX
中科院分区：
文献类型：
--
作者：
Gao Y;Neubauer M;Yang A;Johnson N;Morse M;Li G;Tang JX
鞭毛细菌的运动性主要取决于其细胞器，如鞭毛和皮利，以及外部介质的物理性质，如粘度和基质弹性。我们研究了运动的野生型和两个突变株的新月柄杆菌群细胞在两种不同类型的媒体：粘性和高渗甘油生长培养基的混合物和粘弹性的生长培养基，含有聚乙二醇或聚环氧乙烷的不同定义的大小。对于所有三种菌株在培养基中含有甘油，我们发现线性下降的百分比运动细胞和速度的下降，保持能动的粘度成反比。大多数固定化细胞失去活力，证明了他们的膜泄漏。在粘弹介质中，我们发现在与粘性介质相当的剪切粘度值下，运动性损失较小，游泳速度下降减弱。在这两种类型的媒体中，我们发现更严重的损失的野生型比没有皮利的突变体的运动细胞的百分比，表明干扰的皮利与鞭毛运动加剧粘度增加。然而，我们发现在所有测试条件下，所有三种菌株中保持运动的细胞的游泳速度没有差异。最后，粘弹性介质引起鞭毛电机开关之间的间隔没有显着变化，除非电机失速。高渗效应导致运动性丧失和细胞死亡。向细胞培养基中添加聚合物也会由于剪切粘度增加而导致运动性丧失，但大多数固定化细菌仍保持活力。粘性和粘弹性介质都改变了鞭毛细菌的运动性，而不影响其电机开关行为的内部调节。本文的在线版本（doi：10.1186/s12866-014-0322-3）包含补充材料，可供授权用户使用。
Motility of flagellated bacteria depends crucially on their organelles such as flagella and pili, as well as physical properties of the external medium, such as viscosity and matrix elasticity. We studied the motility of wild-type and two mutant strains of Caulobacter crescentus swarmer cells in two different types of media: a viscous and hyperosmotic glycerol-growth medium mixture and a viscoelastic growth medium, containing polyethylene glycol or polyethylene oxide of different defined sizes. For all three strains in the medium containing glycerol, we found linear drops in percentage of motile cells and decreases in speed of those that remained motile to be inversely proportional to viscosity. The majority of immobilized cells lost viability, evidenced by their membrane leakage. In the viscoelastic media, we found less loss of motility and attenuated decrease of swimming speed at shear viscosity values comparable to the viscous medium. In both types of media, we found more severe loss in percentage of motile cells of wild-type than the mutants without pili, indicating that the interference of pili with flagellated motility is aggravated by increased viscosity. However, we found no difference in swimming speed among all three strains under all test conditions for the cells that remained motile. Finally, the viscoelastic medium caused no significant change in intervals between flagellar motor switches unless the motor stalled. Hyperosmotic effect causes loss of motility and cell death. Addition of polymers into the cell medium also causes loss of motility due to increased shear viscosity, but the majority of immobilized bacteria remain viable. Both viscous and viscoelastic media alter the motility of flagellated bacteria without affecting the internal regulation of their motor switching behavior. The online version of this article (doi:10.1186/s12866-014-0322-3) contains supplementary material, which is available to authorized users.
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影响因子：
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作者：
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