Surface contact stimulates the just-in-time deployment of bacterial adhesins.
Surface contact stimulates the just-in-time deployment of bacterial adhesins.
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DOI：
10.1111/j.1365-2958.2011.07909.x
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发表时间：
2012-01
影响因子：
3.6
通讯作者：
Brun YV
中科院分区：
文献类型：
--
作者：
Li G;Brown PJ;Tang JX;Xu J;Quardokus EM;Fuqua C;Brun YV
细菌附着于表面提供了诸如增加营养获取和对环境压力的抵抗力的优点。附着开始于可逆阶段，通常由表面结构如鞭毛和皮利介导，随后过渡到不可逆附着，通常由多糖介导。在这里，我们表明，皮利和鞭毛旋转之间的相互作用刺激可逆和多糖介导的不可逆附着之间的快速转变。我们发现，新月柄杆菌细胞的可逆附着是由运动细胞轴承皮利介导的，并且它们与表面的接触导致鞭毛旋转的快速皮利依赖性逮捕和极性固着粘附多糖的同时刺激。类似的刺激极性粘附素生产的表面接触发生在Asticcacaulis biprosthecum和根癌农杆菌。因此，单个细菌细胞通过触发即时粘附素的产生来响应它们与表面的初始接触。这种机制将稳定的附着限制在紧密的表面相互作用上，从而使表面附着最大化，阻止非生产性的自粘附，并防止粘合剂固化。
The attachment of bacteria to surfaces provides advantages such as increasing nutrient access and resistance to environmental stress. Attachment begins with a reversible phase, often mediated by surface structures such as flagella and pili, followed by a transition to irreversible attachment, typically mediated by polysaccharides. Here we show that the interplay between pili and flagellum rotation stimulates the rapid transition between reversible and polysaccharide-mediated irreversible attachment. We found that reversible attachment of Caulobacter crescentus cells is mediated by motile cells bearing pili and that their contact with a surface results in the rapid pili-dependent arrest of flagellum rotation and concurrent stimulation of polar holdfast adhesive polysaccharide. Similar stimulation of polar adhesin production by surface contact occurs in Asticcacaulis biprosthecum and Agrobacterium tumefaciens. Therefore, single bacterial cells respond to their initial contact with surfaces by triggering just-in-time adhesin production. This mechanism restricts stable attachment to intimate surface interactions, thereby maximizing surface attachment, discouraging non-productive self-adherence, and preventing curing of the adhesive.
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影响因子：
16
作者：
Paul K;Nieto V;Carlquist WC;Blair DF;Harshey RM
通讯作者：
Harshey RM
影响因子：
3.2
作者：
Bodenmiller, D;Toh, E;Brun, YV
通讯作者：
Brun, YV
影响因子：
2.4
作者：
Hosek, M;Tang, JX
通讯作者：
Tang, JX
影响因子：
5.6
作者：
DEGNEN, ST;NEWTON, A
通讯作者：
NEWTON, A
影响因子：
3.2
作者：
Agladze, K;Wang, X;Romeo, T
通讯作者：
Romeo, T