Cyto- and chemoarchitecture of the hypothalamic paraventricular nucleus in the C57BL/6J male mouse: a study of immunostaining and multiple fluorescent tract tracing.
Cyto- and chemoarchitecture of the hypothalamic paraventricular nucleus in the C57BL/6J male mouse: a study of immunostaining and multiple fluorescent tract tracing.
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DOI：
10.1002/cne.22698
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发表时间：
2012-01-01
影响因子：
2.5
通讯作者：
Dong, Hong-Wei
中科院分区：
文献类型：
--
作者：
Biag, Jonathan;Huang, Yi;Gou, Lin;Hintiryan, Houri;Askarinam, Asal;Hahn, Joel D.;Toga, Arthur W.;Dong, Hong-Wei
下丘脑室旁核（PVH）在自主神经、神经内分泌和行为活动的调节中起着关键作用。这种理解来自于对大鼠PVH的广泛表征，对于这种哺乳动物物种，我们现在有了一个基本PVH神经解剖学和功能的强大模型。然而，在小鼠中，其作为模型研究动物的使用随着遗传操作工具的日益复杂而迅速发展，尚未实现可比较水平的PVH表征。为了解决这个问题，我们采用了多种荧光束示踪和免疫染色技术在几个不同的组合，以确定神经元连接和细胞和化学结构的PVH在常用的C57 BL/6 J雄性小鼠。我们的研究结果揭示了一个独特的组织在小鼠PVH，这是从雄性大鼠的PVH有很大的不同。的差异是特别明显的两个主要的神经内分泌部门（magnocellular和parvicellular）和三个下降preautonomic人口的PVH的空间关系。我们讨论了这些数据有关什么是已知的PVH的功能，并提供了工作作为进一步研究的神经元结构和功能的小鼠PVH的资源。
The paraventricular nucleus of the hypothalamus (PVH) plays a critical role in the regulation of autonomic, neuroendocrine, and behavioral activities. This understanding has come from extensive characterization of the PVH in rats, and for this mammalian species we now have a robust model of basic PVH neuroanatomy and function. However, in mice, whose use as a model research animal has burgeoned with the increasing sophistication of tools for genetic manipulation, a comparable level of PVH characterization has not been achieved. To address this, we employed a variety of fluorescent tract tracing and immunostaining techniques in several different combinations to determine the neuronal connections and cyto- and chemoarchitecture of the PVH in the commonly used C57BL/6J male mouse. Our findings reveal a distinct organization in the mouse PVH that is substantially different from the PVH of male rats. The differences are particularly evident with respect to the spatial relations of two principal neuroendocrine divisions (magnocellular and parvicellular) and three descending preautonomic populations in the PVH. We discuss these data in relation to what is known about PVH function and provide the work as a resource for further studies of the neuronal architecture and function of the mouse PVH.
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影响因子：
16.2
作者：
Fanselow, Michael S.;Dong, Hong-Wei
通讯作者：
Dong, Hong-Wei
影响因子：
2.5
作者：
Braz, Joao M.;Enquist, Lynn W.;Basbaum, Allan I.
通讯作者：
Basbaum, Allan I.
影响因子：
16.2
作者：
Choi, GB;Dong, HW;Anderson, DJ
通讯作者：
Anderson, DJ
影响因子：
--
作者：
ABERCROMBIE, M
通讯作者：
ABERCROMBIE, M
DOI：
10.1002/cne.22283
发表时间：
2010-05-01
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
Geerling JC;Shin JW;Chimenti PC;Loewy AD
通讯作者：
Loewy AD