Potential use of high-throughput sequencing of soil microbial communities for estimating the adverse effects of continuous cropping on ramie (Boehmeria nivea L. Gaud).
Potential use of high-throughput sequencing of soil microbial communities for estimating the adverse effects of continuous cropping on ramie (Boehmeria nivea L. Gaud).
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土壤微生物群落高通量测序在评估连作对苎麻（Boehmeria nivea L. Gaud）的不利影响方面的潜在用途
DOI：
10.1371/journal.pone.0197095
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Dai Q
中科院分区：
文献类型：
--
作者：
Zhu S;Wang Y;Xu X;Liu T;Wu D;Zheng X;Tang S;Dai Q
苎麻（Boehmeria nivea L. Gaud）纤维是最重要的天然纤维之一，是从茎皮中提取的。连作是苎麻茎生长的主要障碍，也是减产的主要原因。根部相关微生物在植物生长和健康中发挥着至关重要的作用。在这项研究中，我们研究了健康和连作的苎麻植物土壤中微生物群落之间的差异，并试图找出这些群落可以抵消连作带来的问题的潜在机制。采用 16S rRNA 和 ITS 基因扩增子的双端 Illumina MiSeq 分析来研究细菌和真菌群落。长期单一种植苎麻会显着降低纤维产量并改变土壤微生物群落。我们的研究结果揭示了微生物群落和功能多样性如何根据种植年份和植物健康状况而变化。土壤细菌多样性随着苎麻单一栽培时期的增加而增加，而真菌则没有观察到显着差异。序列分析表明，厚壁菌门、变形菌门和酸杆菌门是最丰富的细菌门。厚壁菌门丰度随着苎麻单一栽培时间的延长而减少，并与茎长度、茎直径和纤维产量呈正相关。 Actinobacteria、Chloroflexi 和 Zygomycota 门与连续培养期间的产量呈现显着（P < 0.05）负相关。一些放线菌门成员表现出微生物多样性降低，这阻碍了苎麻的连续种植。子囊菌门、接合菌门和担子菌门是主要的真菌门。在健康苎麻中观察到的相对较高丰度的芽孢杆菌可能有助于抑制疾病，从而促进苎麻生长。综上所述，长期连作后苎麻植物土壤软弱、病害增多的原因可能是土壤微生物发生变化，有益微生物减少，有害微生物积累。
Ramie (Boehmeria nivea L. Gaud) fiber, one of the most important natural fibers, is extracted from stem bark. Continuous cropping is the main obstacle to ramie stem growth and a major cause of reduced yields. Root-associated microbes play crucial roles in plant growth and health. In this study, we investigated differences between microbial communities in the soil of healthy and continuously cropped ramie plants, and sought to identify potential mechanisms whereby these communities could counteract the problems posed by continuous cropping. Paired-end Illumina MiSeq analysis of 16S rRNA and ITS gene amplicons was employed to study bacterial and fungal communities. Long-term monoculture of ramie significantly decreased fiber yields and altered soil microbial communities. Our findings revealed how microbial communities and functional diversity varied according to the planting year and plant health status. Soil bacterial diversity increased with the period of ramie monoculture, whereas no significant differences were observed for fungi. Sequence analyses revealed that Firmicutes, Proteobacteria, and Acidobacteria were the most abundant bacterial phyla. Firmicutes abundance decreased with the period of ramie monoculture and correlated positively with the stem length, stem diameter, and fiber yield. The Actinobacteria, Chloroflexi, and Zygomycota phyla exhibited a significant (P < 0.05) negative correlation with yields during continuous cultivation. Some Actinobacteria members showed reduced microbial diversity, which prevented continuous ramie cropping. Ascomycota, Zygomycota, and Basidiomycota were the main fungal phyla. The relatively high abundance of Bacillus observed in healthy ramie may contribute to disease suppression, thereby promoting ramie growth. In summary, soil weakness and increased disease in ramie plants after long-term continuous cropping can be attributed to changes in soil microbes, a reduction in beneficial microbes, and an accumulation of harmful microbes.
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影响因子：
9.7
作者：
Dias, Armando Cavalcante Franco;Hoogwout, Eelco Frans;van Elsas, Jan Dirk
通讯作者：
van Elsas, Jan Dirk
影响因子：
3.6
作者：
Fu, Qinglin;Liu, Chen;Wang, Hailong
通讯作者：
Wang, Hailong
影响因子：
3.7
作者：
Chen MH;Ha EH;Wen TW;Su YN;Lien GW;Chen CY;Chen PC;Hsieh WS
通讯作者：
Hsieh WS
影响因子：
4.4
作者：
Filion, M;Hamelin, RC;St-Arnaud, M
通讯作者：
St-Arnaud, M
影响因子：
9.7
作者：
Jangid, Kamlesh;Williams, Mark A.;Whitman, William B.
通讯作者：
Whitman, William B.