A transgenic male-only strain of the New World screwworm for an improved control program using the sterile insect technique.
A transgenic male-only strain of the New World screwworm for an improved control program using the sterile insect technique.
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DOI：
10.1186/s12915-016-0296-8
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发表时间：
2016-08-30
期刊：
影响因子：
5.4
通讯作者：
Scott MJ
中科院分区：
文献类型：
--
作者：
Concha C;Palavesam A;Guerrero FD;Sagel A;Li F;Osborne JA;Hernandez Y;Pardo T;Quintero G;Vasquez M;Keller GP;Phillips PL;Welch JB;McMillan WO;Skoda SR;Scott MJ
新世界螺旋虫（Cochliomyia hominivorax）是西半球亚热带和热带地区特有的毁灭性牲畜害虫。该物种的幼虫以包括人类在内的活体动物的组织为食，如果不治疗可能导致死亡。60多年前，昆虫不育技术（SIT）被开发出来，目的是根除这种害虫，最初来自佛罗里达，但随后来自整个北美和中美洲。从一开始，人们就认识到，如果只在田间释放不育的雄性，SIT会更有效，但直到现在这是不可能的。在这里，我们报告的发展和评价的第一性别鉴定菌株的C。在没有四环素的食物中饲养时只产生雄性的食人动物。已经开发出具有四环素抑制的雌性致死遗传系统的转基因系。其中10个品系在幼虫/蛹后期表现出较高的雌性致死率，其中3个品系表现出显性雌性致死率。大多数品系在与生产设施中大量饲养相关的几个适应性参数方面与野生型亲本品系相当。此外，三个线表现良好，在男性交配成功和男性竞争测定，这表明他们将在该领域的性竞争。因此，新世界螺旋虫计划选择了一个转基因品系用于在大量饲养条件下进行评估。我们的结论是，选择的性别鉴定菌株的有前途的特性可能有助于降低现有根除计划的生产成本，并提供更有效的种群抑制，这应该使遗传控制计划在C.食人动物仍然是地方病。本文的在线版本（doi：10.1186/s12915-016-0296-8）包含补充材料，可供授权用户使用。
The New World screwworm, Cochliomyia hominivorax, is a devastating pest of livestock endemic to subtropical and tropical regions of the Western hemisphere. The larvae of this species feed on the tissue of living animals, including man, and can cause death if untreated. Over 60 years ago, the sterile insect technique (SIT) was developed with the aim of eradicating this pest, initially from Florida but subsequently from all of North and Central America. From the outset it was appreciated that SIT would be more efficient if only sterile males were released in the field, but this was not possible until now. Here, we report on the development and evaluation of the first sexing strains of C. hominivorax that produce only males when raised on diet without tetracycline. Transgenic lines have been developed that possess a tetracycline repressible female-lethal genetic system. Ten of these lines show high female lethality at the late larval/pupal stages and three of them present dominant female lethality. Most of the lines were comparable to the wild type parental strain in several fitness parameters that are relevant to mass rearing in a production facility. Further, three lines performed well in male mating success and male competition assays, suggesting they would be sexually competitive in the field. Consequently, one transgenic line has been selected by the New World Screwworm Program for evaluation under mass rearing conditions. We conclude that the promising characteristics of the selected sexing strains may contribute to reduce production costs for the existing eradication program and provide more efficient population suppression, which should make a genetic control program more economical in regions were C. hominivorax remains endemic. The online version of this article (doi:10.1186/s12915-016-0296-8) contains supplementary material, which is available to authorized users.
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影响因子：
3.8
作者：
Carvalho DO;McKemey AR;Garziera L;Lacroix R;Donnelly CA;Alphey L;Malavasi A;Capurro ML
通讯作者：
Capurro ML
影响因子：
5.4
作者：
Phuc HK;Andreasen MH;Burton RS;Vass C;Epton MJ;Pape G;Fu G;Condon KC;Scaife S;Donnelly CA;Coleman PG;White-Cooper H;Alphey L
通讯作者：
Alphey L
影响因子：
46.9
作者：
Harris, Angela F.;Nimmo, Derric;Alphey, Luke
通讯作者：
Alphey, Luke
影响因子：
46.9
作者：
Gong, P;Epton, MJ;Alphey, L
通讯作者：
Alphey, L
影响因子：
46.9
作者：
Fu, Guoliang;Condon, Kirsty C.;Alphey, Luke
通讯作者：
Alphey, Luke