MRI-guided dissection of the nonhuman primate brain: a case study.
MRI-guided dissection of the nonhuman primate brain: a case study.
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DOI：
10.1016/j.ymeth.2009.03.023
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发表时间：
2010-03
期刊：
影响因子：
4.8
通讯作者：
Friedman, David Paul
中科院分区：
文献类型：
--
作者：
Daunais, James Bernard;Kraft, Robert Arthur;Davenport, April Teresa;Burnett, Elizabeth J.;Maxey, Vicki Moser;Szeliga, Kendall Thomas;Rau, Andrew Ryan;Flory, Graham Stallard;Hemby, Scott Edwards;Kroenke, Christopher David;Grant, Kathleen Alice;Friedman, David Paul
许多生物化学和电生理学技术需要在死亡后必须非常迅速地取回组织，以保持神经元环境的生理完整性。因此，准确预测大脑感兴趣区域（ROI）的精确位置并在大脑采集后尽快检索这些区域的能力对于后续分析至关重要。实现这一目标的一种方法是利用高分辨率MRI扫描来指导随后的解剖。在本研究中，采用长期自我给药乙醇的恒河猴和食蟹猴的大脑的个体MRI扫描，以确定哪些解剖组织块包含特定的ROI。MRI引导的离散脑区脑解剖在100%的病例中完全准确。相比之下，仅依靠外部标志而不借助MRI的解剖实现了约60-70%的准确性。这些结果清楚地表明，高分辨率MR成像可以提高靶向特定脑区的准确性。
Numerous biochemical as well as electrophysiological techniques require tissue that must be retrieved very quickly following death in order to preserve the physiological integrity of the neuronal environment. Therefore, the ability to accurately predict the precise locations of brain regions of interest (ROI) and to retrieve those areas as quickly as possible following the brain harvest is critical for subsequent analyses. One way to achieve this objective is the utilization of high resolution MRI scans to guide the subsequent dissections. In the present study, individual MRI scans of the brains of rhesus and cynomolgus macaques that had chronically self-administered ethanol were employed in order to determine which blocks of dissected tissue contained specific ROIs. MRI-guided brain dissection of discrete brain regions was completely accurate in 100% of the cases. In comparison, approximately 60–70% accuracy was achieved in dissections that relied on external landmarks alone without the aid of MRI. These results clearly demonstrate that the accuracy of targeting specific brain areas can be improved with high-resolution MR imaging.
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影响因子：
3
作者：
Deogaonkar, M;Heers, M;Subramanian, T
通讯作者：
Subramanian, T
DOI：
10.1124/jpet.104.072025
发表时间：
2004-12-01
影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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