Diagnosis of invasive amebiasis by enzyme-linked immunosorbent assay of saliva to detect amebic lectin antigen and anti-lectin immunoglobulin G antibodies.
Diagnosis of invasive amebiasis by enzyme-linked immunosorbent assay of saliva to detect amebic lectin antigen and anti-lectin immunoglobulin G antibodies.
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通过唾液酶联免疫吸附测定检测阿米巴凝集素抗原和抗凝集素免疫球蛋白 G 抗体诊断侵袭性阿米巴病。
DOI：
10.1128/jcm.38.6.2344-2347.2000
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发表时间：
2000
影响因子：
9.4
通讯作者：
Ravdin,JI
中科院分区：
文献类型：
--
作者：
Abd-Alla,MD;Jackson,TF;Reddy,S;Ravdin,JI
用酶联免疫吸附试验（ELISA）检测阿米巴肝脓肿（ALA）、急性阿米巴结肠炎、溶组织内阿米巴无症状感染者和未感染者的唾液中是否存在E.溶组织半乳糖凝集素抗原和唾液免疫球蛋白（IgG）抗体，以重组富含半胱氨酸的凝集素衍生蛋白（LC 3）。唾液凝集素抗原在急性结肠炎患者中的检出率为65.8%，而在ALA恢复期患者中的检出率为22.2%，在无症状结肠炎患者中的检出率为10.0%。溶组织感染率为9.8%。大肠杆菌感染，对照组（来自美国的受试者和患有非阿米巴腹泻的研究患者）为2.6%（与结肠炎受试者的值相比，每个受试者的P< 0.001）。在92%的ALA患者中发现了唾液抗LC3 IgG抗体，无论疾病持续时间如何，在83.3%的结肠炎患者中发现了至少7天的症状（与其他研究组相比，P< 0.001）。在56.3%的急性结肠炎受试者、100%的ALA或长期结肠炎受试者、45%的无症状结肠炎受试者中检测到血清抗LC 3 IgG抗体。溶组织性大肠杆菌感染率为32.3%。腹泻控制率为23.4%。与血清抗LC3 IgG抗体的ELISA相比，唾液凝集素抗原测定是急性阿米巴结肠炎的更敏感和特异性的试验。ELISA法检测唾液中抗LC3 IgG抗体是诊断ALA和迁延性阿米巴结肠炎的有效手段。
Saliva from subjects with amebic liver abscess (ALA), acute amebic colitis, asymptomatic infection withEntamoeba histolyticaorEntamoeba dispar, and uninfected controls was tested by enzyme-linked immunosorbent assay (ELISA) for the presence ofE. histolyticagalactose-inhibitable lectin antigen and salivary immunoglobulin (IgG) antibodies to a recombinant cysteine-rich lectin-derived protein (LC3). Salivary lectin antigen was found in 65.8% of subjects with acute colitis, compared to 22.2% of those convalescent from ALA, 10.0% with asymptomaticE. histolyticainfection, 9.8% withE. disparinfection, and 2.6% of controls (subjects from the United States and study patients with nonamebic diarrhea) (P< 0.001 for each compared to values for subjects with colitis). Salivary anti-LC3 IgG antibodies were found in 92% of ALA patients regardless of duration of illness and in 83.3% of colitis patients who were symptomatic for at least 7 days (P< 0.001 compared to other study groups). Serum anti-LC3 IgG antibodies were detected in 56.3% of subjects with acute colitis, 100% of subjects with ALA or prolonged colitis, 45% of subjects with asymptomaticE. histolyticainfection, 32.3% of subjects withE. disparinfection, and 23.4% of diarrhea controls. In comparison to ELISA for serum anti-LC3 IgG antibodies, the salivary lectin antigen assay is a more sensitive and specific test for acute amebic colitis. Detection of salivary anti-LC3 IgG antibodies by ELISA is an effective means for the diagnosis of ALA and prolonged cases of amebic colitis.
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