Controlling the stoichiometry and strand polarity of a tetramolecular G-quadruplex structure by using a DNA origami frame.
Controlling the stoichiometry and strand polarity of a tetramolecular G-quadruplex structure by using a DNA origami frame.
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DOI：
10.1093/nar/gkt592
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发表时间：
2013-10
影响因子：
14.9
通讯作者：
Sugiyama H
中科院分区：
文献类型：
--
作者：
Rajendran A;Endo M;Hidaka K;Tran PL;Mergny JL;Sugiyama H
富含鸟嘌呤的寡核苷酸往往表现出形成超分子结构的强烈倾向，即所谓的g -四重结构。由于其生物学意义，它现在被认为是DNA最重要的构象之一。在这里，我们描述了在KCl溶液中形成四分子g -四复体的直接可视化和单分子分析。构象变化是通过将两个双工DNA（中间有G-G错配重复序列）放入DNA折纸框架中并监测链的拓扑变化来实现的。在没有KCl的情况下，合并的双相化合物没有相互作用，彼此平行排列。KCl的加入诱导了g -四重体结构的形成，使其在中间稳定地相互结合。这样的四重结构允许DNA突触在不干扰参与序列的双工区域的情况下发生，并导致原子力显微镜监测到的x形结构。此外，实时分析了KCl溶液中g -四聚体的形成及其在无KCl缓冲液中的破坏。g -四联体的取向通常难以用传统的生化方法来控制和研究。然而，我们使用DNA折纸的方法可以成功地控制g -四重体的链取向、拓扑结构和化学计量。
Guanine-rich oligonucleotides often show a strong tendency to form supramolecular architecture, the so-called G-quadruplex structure. Because of the biological significance, it is now considered to be one of the most important conformations of DNA. Here, we describe the direct visualization and single-molecule analysis of the formation of a tetramolecular G-quadruplex in KCl solution. The conformational changes were carried out by incorporating two duplex DNAs, with G–G mismatch repeats in the middle, inside a DNA origami frame and monitoring the topology change of the strands. In the absence of KCl, incorporated duplexes had no interaction and laid parallel to each other. Addition of KCl induced the formation of a G-quadruplex structure by stably binding the duplexes to each other in the middle. Such a quadruplex formation allowed the DNA synapsis without disturbing the duplex regions of the participating sequences, and resulted in an X-shaped structure that was monitored by atomic force microscopy. Further, the G-quadruplex formation in KCl solution and its disruption in KCl-free buffer were analyzed in real-time. The orientation of the G-quadruplex is often difficult to control and investigate using traditional biochemical methods. However, our method using DNA origami could successfully control the strand orientations, topology and stoichiometry of the G-quadruplex.
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影响因子：
16.6
作者：
通讯作者：
--
DOI：
10.1143/jjap.41.4851
发表时间：
2002-07-01
期刊：
JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS
影响因子：
--
作者：
Ando, T;Kodera, N;Toda, A
通讯作者：
Toda, A
DOI：
10.1073/pnas.211400898
发表时间：
2001-10-23
影响因子：
11.1
作者：
Ando, T;Kodera, N;Toda, A
通讯作者：
Toda, A
影响因子：
38.3
作者：
通讯作者：
--
影响因子：
3.2
作者：
Müller S;Sanders DA;Di Antonio M;Matsis S;Riou JF;Rodriguez R;Balasubramanian S
通讯作者：
Balasubramanian S