Developmental expression of nitric oxide/cyclic GMP synthesizing cells in the nervous system of Drosophila melanogaster.
Developmental expression of nitric oxide/cyclic GMP synthesizing cells in the nervous system of Drosophila melanogaster.
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果蝇神经系统中一氧化氮/环鸟苷酸合成细胞的发育表达。
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Gerd Bicker
中科院分区：
文献类型：
--
作者：
B. Wildemann;Gerd Bicker
一氧化氮（NO）是一种通过细胞膜渗透的信号分子，它可以激活可溶性的鸟酰环化酶并导致环状GMP （cGMP）的形成。NO/cGMP信号系统被认为在脊椎动物和无脊椎动物的发育过程中起着重要作用。在此，我们分析了黑腹果蝇神经系统发育过程中该信号通路的细胞表达。利用NADPH diaphorase组织化学作为NO合成酶的标记物，我们确定了几种神经元和胶质细胞类型作为潜在的NO供体细胞。为了标记no应答的靶细胞，我们采用免疫细胞化学技术检测cGMP。NO供体组织的孵育诱导cGMP免疫反应性（cGMP- ir）在单个运动神经元、感觉神经元和脑和腹侧神经索的中间神经元群。NADPHd染色和cGMP-IR在胚胎期、幼虫期和蛹前期的细胞表达呈动态变化。NADPH降脂酶和cGMP-IR在幼虫中枢神经系统（CNS）不同神经元群中的表达表明，NO在中枢神经系统内的跨细胞信号传导中起作用，并作为神经肌肉连接处的潜在逆行信使。此外，含有NO反应感觉神经元的NADPH diaphorase阳性影像盘的存在表明，跨细胞NO/cGMP信使系统可以在上皮细胞和神经元表型之间运作。在可识别的细胞类型中对供体和NO应答靶细胞进行离散细胞分辨，将有助于对发育中的果蝇神经系统中NO/cGMP信号转导的遗传学、药理学和生理学分析。
Nitric oxide (NO) is a membrane-permeant signaling molecule which activates soluble guanylyl cyclase and leads to the formation of cyclic GMP (cGMP). The NO/cGMP signaling system is thought to play essential roles during the development of vertebrate and invertebrate animals. Here, we analyzed the cellular expression of this signaling pathway during the development of the Drosophila melanogaster nervous system. Using NADPH diaphorase histochemistry as a marker for NO synthase, we identified several neuronal and glial cell types as potential NO donor cells. To label NO-responsive target cells, we used the detection of cGMP by an immunocytochemical technique. Incubation of tissue in an NO donor induced cGMP immunoreactivity (cGMP-IR) in individual motoneurons, sensory neurons, and groups of interneurons of the brain and ventral nerve cord. A dynamic pattern of the cellular expression of NADPHd staining and cGMP-IR was observed during embryonic, larval, and prepupal phases. The expression of NADPH diaphorase and cGMP-IR in distinct neuronal populations of the larval central nervous system (CNS) indicates a role of NO in transcellular signaling within the CNS and as potential retrograde messenger across the neuromuscular junction. In addition, the presence of NADPH diaphorase-positive imaginal discs containing NO-responsive sensory neurons suggests that a transcellular NO/cGMP messenger system can operate between cells of epithelial and neuronal phenotype. The discrete cellular resolution of donor and NO-responsive target cells in identifiable cell types will facilitate the genetic, pharmacological, and physiological analysis of NO/cGMP signal transduction in the developing nervous system of Drosophila.
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影响因子：
56.9
作者：
H. H. Wu-H.;C. Williams;S. McLoon
通讯作者：
H. H. Wu-H.;C. Williams;S. McLoon
影响因子：
56.9
作者：
VERMA, A;HIRSCH, DJ;SNYDER, SH
通讯作者：
SNYDER, SH
影响因子：
4.6
作者：
Gerard Campbell;Harald H.H. Göring;Tina Lin;E. Spana;S. Andersson;C. Doe;Andrew Tomlinson
通讯作者：
Gerard Campbell;Harald H.H. Göring;Tina Lin;E. Spana;S. Andersson;C. Doe;Andrew Tomlinson
一氧化氮敏感的鸟苷酸环化酶活性与昆虫神经元发育的成熟期相关。
DOI：
10.1242/dev.122.12.3949
发表时间：
1996
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Truman,JW;DeVente,J;Ball,EE
通讯作者：
Ball,EE
DOI：
10.1073/pnas.92.20.9072
发表时间：
1995-09-26
影响因子：
11.1
作者：
REGULSKI, M;TULLY, T
通讯作者：
TULLY, T