The Anaerobic Product Ethanol Promotes Autophagy-Dependent Submergence Tolerance in Arabidopsis.
The Anaerobic Product Ethanol Promotes Autophagy-Dependent Submergence Tolerance in Arabidopsis.
复制标题
厌氧产物乙醇促进拟南芥自噬依赖性淹没耐受性。
DOI：
10.3390/ijms21197361
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发表时间：
2020-10-05
影响因子：
5.6
通讯作者：
Xie LJ
中科院分区：
文献类型：
--
作者：
Yuan LB;Chen L;Zhai N;Zhou Y;Zhao SS;Shi LL;Xiao S;Yu LJ;Xie LJ
为了应对淹没条件下的缺氧，植物从有氧呼吸转变为厌氧发酵，这导致最终产物乙醇的积累。我们以前报道过拟南芥自噬缺陷突变体对乙醇处理的敏感性增加，表明乙醇可能参与调节自噬介导的缺氧反应。在这里，使用转录组学分析，我们确定了3909个基因在拟南芥幼苗的差异表达响应乙醇处理，包括2487上调和1422下调基因。乙醇处理显著上调了参与自噬和活性氧解毒的基因。使用表达融合于绿色荧光蛋白的自体吞噬相关蛋白8 e（GFP-ATG 8 e）的转基因系，我们证实了外源性乙醇处理促进体内自体吞噬体的形成。表型分析表明，在adh 1突变体中的乙醇脱氢酶基因的缺失导致在减弱的耐淹性，减少积累的ATG蛋白，减少淹没诱导的自噬体的形成。与耐淹拟南芥哥伦比亚（Col-0）相比，不耐淹的兰茨贝格erecta（Ler）对乙醇处理表现出超敏反应;我们将这些表型与ADH 1功能的差异和这些加入之间的自噬机制联系起来。因此，乙醇促进自噬介导的耐淹拟南芥。
In response to hypoxia under submergence, plants switch from aerobic respiration to anaerobic fermentation, which leads to the accumulation of the end product, ethanol. We previously reported that Arabidopsis thaliana autophagy-deficient mutants show increased sensitivity to ethanol treatment, indicating that ethanol is likely involved in regulating the autophagy-mediated hypoxia response. Here, using a transcriptomic analysis, we identified 3909 genes in Arabidopsis seedlings that were differentially expressed in response to ethanol treatment, including 2487 upregulated and 1422 downregulated genes. Ethanol treatment significantly upregulated genes involved in autophagy and the detoxification of reactive oxygen species. Using transgenic lines expressing AUTOPHAGY-RELATED PROTEIN 8e fused to green fluorescent protein (GFP-ATG8e), we confirmed that exogenous ethanol treatment promotes autophagosome formation in vivo. Phenotypic analysis showed that deletions in the alcohol dehydrogenase gene in adh1 mutants result in attenuated submergence tolerance, decreased accumulation of ATG proteins, and diminished submergence-induced autophagosome formation. Compared to the submergence-tolerant Arabidopsis accession Columbia (Col-0), the submergence-intolerant accession Landsberg erecta (Ler) displayed hypersensitivity to ethanol treatment; we linked these phenotypes to differences in the functions of ADH1 and the autophagy machinery between these accessions. Thus, ethanol promotes autophagy-mediated submergence tolerance in Arabidopsis.
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影响因子：
7.4
作者：
Bailey-Serres, Julia;Lee, Seung Cho;Brinton, Erin
通讯作者：
Brinton, Erin
自噬有助于调节拟南芥淹没过程中的缺氧反应
影响因子：
13.3
作者：
Chen L;Liao B;Qi H;Xie LJ;Huang L;Tan WJ;Zhai N;Yuan LB;Zhou Y;Yu LJ;Chen QF;Shu W;Xiao S
通讯作者：
Xiao S
绿色生物的低氧反应机制。
影响因子：
5.6
作者：
Banti V;Giuntoli B;Gonzali S;Loreti E;Magneschi L;Novi G;Paparelli E;Parlanti S;Pucciariello C;Santaniello A;Perata P
通讯作者：
Perata P
DOI：
10.1111/j.1530-0277.2012.01742.x
发表时间：
2012-08
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
Dolganiuc A;Thomes PG;Ding WX;Lemasters JJ;Donohue TM Jr
通讯作者：
Donohue TM Jr
影响因子：
7.4
作者：
Giuntoli, Beatrice;Perata, Pierdomenico
通讯作者：
Perata, Pierdomenico