Short tandem repeat profiling: part of an overall strategy for reducing the frequency of cell misidentification.
Short tandem repeat profiling: part of an overall strategy for reducing the frequency of cell misidentification.
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DOI：
10.1007/s11626-010-9352-9
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发表时间：
2010-12
影响因子：
2.1
通讯作者：
Elmore, Eugene
中科院分区：
文献类型：
--
作者：
Nims, Raymond W.;Sykes, Greg;Cottrill, Karin;Ikonomi, Pranvera;Elmore, Eugene
细胞认证在生物医学科学中的作用受到了相当多的关注，特别是在过去的十年里。这种质量控制属性现在开始受到资助机构和科学期刊的重视。短串联重复 (STR) 分析是目前可用的少数 DNA 分析技术之一，被提议用于人类细胞系、干细胞和组织的常规鉴定（验证）。与同工酶分析、核型分析、人类白细胞抗原分型等方法相比，该技术的优点在于，只要评估适当数量和类型的基因座，STR 分析就可以建立个体水平的同一性。为了最好地利用这项技术，需要一个标准化的协议和一个数据驱动、质量控制和可公开搜索的数据库。这个公共 STR 数据库（目前正在开发）将使研究人员能够快速验证细胞来源个体的人类培养物。对非人类动物细胞使用类似的方法将需要开发其他合适的基因座集。虽然在更常规的基础上实施 STR 分析应该会显着降低细胞错误识别的频率，但可能需要额外的技术作为整体身份验证范例的一部分。例如，同工酶分析、基于 PCR 的 DNA 扩增和基于序列的条形码方法可以快速确认细胞系的物种来源，同时筛选交叉污染，特别是当物种特异性 STR 方法无法识别存在的细胞时。在建立 STR 数据库期间，可能还需要核型分析作为支持工具。最后，良好的细胞培养实践必须始终是减少细胞错误识别频率的任何努力的主要组成部分。
The role of cell authentication in biomedical science has received considerable attention, especially within the past decade. This quality control attribute is now beginning to be given the emphasis it deserves by granting agencies and by scientific journals. Short tandem repeat (STR) profiling, one of a few DNA profiling technologies now available, is being proposed for routine identification (authentication) of human cell lines, stem cells, and tissues. The advantage of this technique over methods such as isoenzyme analysis, karyotyping, human leukocyte antigen typing, etc., is that STR profiling can establish identity to the individual level, provided that the appropriate number and types of loci are evaluated. To best employ this technology, a standardized protocol and a data-driven, quality-controlled, and publically searchable database will be necessary. This public STR database (currently under development) will enable investigators to rapidly authenticate human-based cultures to the individual from whom the cells were sourced. Use of similar approaches for non-human animal cells will require developing other suitable loci sets. While implementing STR analysis on a more routine basis should significantly reduce the frequency of cell misidentification, additional technologies may be needed as part of an overall authentication paradigm. For instance, isoenzyme analysis, PCR-based DNA amplification, and sequence-based barcoding methods enable rapid confirmation of a cell line’s species of origin while screening against cross-contaminations, especially when the cells present are not recognized by the species-specific STR method. Karyotyping may also be needed as a supporting tool during establishment of an STR database. Finally, good cell culture practices must always remain a major component of any effort to reduce the frequency of cell misidentification.
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影响因子：
4.8
作者：
Parson, W;Kirchebner, R;Kofler, R
通讯作者：
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影响因子：
14.8
作者：
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通讯作者：
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影响因子：
6.4
作者：
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影响因子：
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作者：
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通讯作者：
Haugen, Bryan R.
DOI：
10.1073/pnas.121616198
发表时间：
2001-07-03
影响因子：
11.1
作者：
Masters, JR;Thomson, JA;Debenham, PG
通讯作者：
Debenham, PG