Metabolome profiling of stratified seeds provides insight into the regulation of dormancy in Davidia involucrata.
Metabolome profiling of stratified seeds provides insight into the regulation of dormancy in Davidia involucrata.
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分层种子的代谢组分析提供了对珙桐休眠调节的深入了解
DOI：
10.1016/j.pld.2021.12.001
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发表时间：
2022-07
期刊：
影响因子：
4.8
通讯作者：
Luo, Shijia
中科院分区：
文献类型：
--
作者：
Deng, Shiming;Xiao, Qiang;Xu, Cigui;Hong, Jian;Deng, Zhijun;Jiang, Dan;Luo, Shijia
珙桐（Davidia involucrata）是一种第三纪的遗迹植物，对寒冷环境有很好的适应性。雪莲种子的休眠持续时间极长，通常在3至4年之间，这一特性使该物种成为研究种子休眠机制的极好模型。天山雪莲种子萌发控制的分子机制至今仍不清楚。据报道，种子层积可以提高柠檬种子的发芽率。我们进行了广泛针对性的代谢物组分析，以确定代谢物和相关的途径，从六个不同的湿砂层积时间（0-30个月），使用超高效液相色谱-Q精确轨道质谱。随着层积时间的延长（12-30个月），发芽率增加。此外，我们在分层种子中检测到10，008种代谢物。我们还检测到48个差异积累的代谢产物（DAMs）之间的所有分层期间的种子，10个高度保守的代谢产物。未层化种子和层化种子之间的大部分差异积累的代谢物富集在嘌呤代谢、嘧啶代谢、黄酮和黄酮醇生物合成、苯丙素类生物合成和精氨酸生物合成途径中。脱落酸、吲哚-3-乙酸和芥子酸等主要植物激素在天山雪莲种子中的积累差异较大，并被预测可能对天山雪莲的休眠起调节作用。我们提供了广泛的代谢信息，对未来的工作，鸽子树发芽研究有用。湿润层积促进珙桐种子萌发。潮湿的层积改变了发芽种子的代谢组。脱落酸、吲哚-3-乙酸和芥子酸调节天山雪莲的休眠。
Dove tree (Davidia involucrata), a tertiary vestige species, is well-adapted to cool conditions. Dormancy in D. involucrata seed lasts for an extremely long period of time, typically between 3 and 4 years, and this characteristic makes the species an excellent model for studying the mechanisms of seed dormancy. The molecular mechanisms governing germination control in D. involucrata are still unknown. Seed stratification have been reported to enhance germination in recalcitrant seeds. We performed a widely targeted metabolome profiling to identify metabolites and associated pathways in D. involucrata seeds from six different moist sand stratification durations (0–30 months) using the ultra-high-performance liquid chromatography-Q Exactive Orbitrap-Mass spectrometry. There was an increasing germination rate with prolonged stratification durations (12–30 months). Furthermore, we detected 10,008 metabolites in the stratified seeds. We also detected 48 differentially accumulated metabolites (DAMs) between all stratification periods in the seeds, with 10 highly conserved metabolites. Most of the differentially accumulated metabolites between unstratified and stratified seeds were enriched in purine metabolism, pyrimidine metabolism, flavone and flavonol biosynthesis, phenylpropanoid biosynthesis, and arginine biosynthesis pathways. Key phytohormones, abscisic acid, indole-3 acetic acid, and sinapic acid were differentially accumulated in the seeds and are predicted to regulate dormancy in D. involucrata. We have provided extensive metabolic information useful for future works on dove tree germination study. Moist stratification improves Davidia involucrata seed germination. Moist stratification alters the metabolome in germinating seeds. Abscisic acid, indole-3 acetic acid, and sinapic acid regulate dormancy in D. involucrata.
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芥子酸或其衍生物干扰拟南芥种子萌发过程中脱落酸稳态
影响因子：
5.3
作者：
Bi B;Tang J;Han S;Guo J;Miao Y
通讯作者：
Miao Y
影响因子：
5.6
作者：
Das A;Kim DW;Khadka P;Rakwal R;Rohila JS
通讯作者：
Rohila JS
影响因子：
6.9
作者：
Cornelius S;Witz S;Rolletschek H;Möhlmann T
通讯作者：
Möhlmann T
影响因子：
7.2
作者：
Brady, SM;Sarkar, SF;McCourt, P
通讯作者：
McCourt, P
DOI：
10.1556/aagr.54.2006.4.7
发表时间：
2006-12-01
期刊：
Acta Agronomica Hungarica
影响因子：
--
作者：
El-Araby, M. M.;Moustafa, S. M. A.;Hegazi, A. Z. A.
通讯作者：
Hegazi, A. Z. A.