Stabilization of Murine Norovirus by Bacteria.
Stabilization of Murine Norovirus by Bacteria.
复制标题
作者：
肠道病毒在宿主体内遇到各种细菌，这会影响感染的结果。诺如病毒与肠道细菌之间的相互作用尚不清楚。先前的工作确定，与常规小鼠相比，小鼠诺如病毒（MNV），一种模型诺如病毒，在抗生素治疗的小鼠中减少了复制。虽然这表明微生物群促进了MNV感染，但其机制尚不完全清楚。此外，先前对其他肠道病毒（如脊髓灰质炎病毒和柯萨奇病毒B3）的研究表明，病毒粒子与细菌结合，接触细菌可以稳定病毒颗粒并限制RNA的过早释放。因此，我们研究了MNV与特定细菌之间的相互作用以及这些相互作用的后果。我们发现大多数革兰氏阳性菌稳定了MNV，而革兰氏阴性菌不稳定MNV。革兰氏阳性和革兰氏阴性细菌都与MNV结合。然而，细菌单独结合不足以稳定病毒粒子，因为革兰氏阴性细菌结合MNV但不稳定病毒粒子。此外，我们发现细菌条件培养基也稳定了MNV，这种稳定可能是由于一个小的热稳定分子。总的来说，这项工作确定了稳定MNV并可能影响环境中病毒粒子稳定性的特定细菌和细菌成分。肠道病毒暴露于肠道内各种各样的细菌中，但细菌对病毒颗粒的影响尚不完全清楚。我们发现，在几种革兰氏阳性菌株的存在下，小鼠诺如病毒（MNV）病毒粒子的稳定性得到增强。病毒稳定活性也存在于细菌培养基中，并在加热或蛋白酶处理下保持活性。这些结果表明，某些细菌和细菌产物可能促进MNV在环境中的稳定性，从而影响病毒的传播。
Enteric viruses encounter various bacteria in the host, which can impact infection outcomes. The interactions between noroviruses and enteric bacteria are not well understood. Previous work determined that murine norovirus (MNV), a model norovirus, had decreased replication in antibiotic-treated mice compared with conventional mice. Although this suggests that the microbiota promotes MNV infection, the mechanisms are not completely understood. Additionally, prior work with other enteric viruses, such as poliovirus and coxsackievirus B3, demonstrated that virions bind bacteria, and exposure to bacteria stabilizes viral particles and limits premature RNA release. Therefore, we examined interactions between MNV and specific bacteria and the consequences of these interactions. We found that the majority of Gram-positive bacteria tested stabilized MNV, while Gram-negative bacteria did not stabilize MNV. Both Gram-positive and Gram-negative bacteria bound to MNV. However, bacterial binding alone was not sufficient for virion stabilization, since Gram-negative bacteria bound MNV but did not stabilize virions. Additionally, we found that bacteria conditioned medium also stabilized MNV and this stabilization may be due to a small heat-stable molecule. Overall, this work identifies specific bacteria and bacterial components that stabilize MNV and may impact virion stability in the environment. IMPORTANCE Enteric viruses are exposed to a wide variety of bacteria in the intestine, but the effects of bacteria on viral particles are incompletely understood. We found that murine norovirus (MNV) virion stability is enhanced in the presence of several Gram-positive bacterial strains. Virion-stabilizing activity was also present in bacterial culture medium, and activity was retained upon heat or protease treatment. These results suggest that certain bacteria and bacterial products may promote MNV stability in the environment, which could influence viral transmission.
登录
查看更多内容
DOI：
10.3390/v13112162
发表时间：
2021-10-26
期刊：
Viruses
影响因子：
--
作者：
Sherman MB;Williams AN;Smith HQ;Pettitt BM;Wobus CE;Smith TJ
通讯作者：
Smith TJ
影响因子：
56.9
作者：
Karst, SM;Wobus, CE;Virgin, HW
通讯作者：
Virgin, HW
影响因子：
5.4
作者：
Dhalech, Adeeba H.;Fuller, Tara D.;Robinson, Christopher M.
通讯作者：
Robinson, Christopher M.
影响因子：
11.8
作者：
Patel, Manish M.;Widdowson, Marc-Alain;Glass, Roger I.;Akazawa, Kenichiro;Vinje, Jan;Parashar, Umesh D.
通讯作者：
Parashar, Umesh D.
影响因子：
6.7
作者：
Berger AK;Yi H;Kearns DB;Mainou BA
通讯作者：
Mainou BA