Three β-Glucuronosyltransferase Genes Involved in Arabinogalactan Biosynthesis Function in Arabidopsis Growth and Development.
Three β-Glucuronosyltransferase Genes Involved in Arabinogalactan Biosynthesis Function in Arabidopsis Growth and Development.
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DOI：
10.3390/plants10061172
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发表时间：
2021-06-09
期刊：
影响因子：
--
通讯作者：
Showalter AM
中科院分区：
文献类型：
--
作者：
Ajayi OO;Held MA;Showalter AM
阿拉伯半乳聚糖蛋白（AGPs）含有与植物生长过程生物学相关的阿拉伯半乳聚糖（AG）多糖。本研究利用glcat 14单突变体、双突变体和三突变体进一步研究了拟南芥中3个位于高尔基体的β-葡萄糖醛酸转移酶基因（GLCAT 14 A、GLCAT 14 B和GLCAT 14 C）的生理生化作用。这些蛋白质在本氏烟草中瞬时表达时定位于高尔基体。从拟南芥茎，叶和长角果的AGP提取物的糖分析表明，在glcat 14突变体相对于野生型的葡萄糖醛酸的一致减少，伴随的影响导致组织特异性的改变，特别是在阿拉伯糖和半乳糖糖。虽然我们观察到glca 14 a/B和glca 14 a/B/c突变体中的毛状体分支缺陷，但扫描电子显微镜分析/能量色散微分析（SEM/EDX）显示，这些突变体中的毛状体的钙含量相对于野生型没有差异。茎和叶的免疫印迹分析显示，相对于野生型，在glcat 14 a/B和glcat 14 a/B/c突变体中用LM 2抗体检测到AGP减少。目前的工作证实了对细胞壁生物合成基因进行结构-功能评估以确定其在植物生长发育中的生理作用的可能性。
Arabinogalactan proteins (AGPs) contain arabinogalactan (AG) polysaccharides that are biologically relevant to plant growth processes. Here, the biochemical and physiological roles of three Golgi localized β-glucuronosyltransferase genes (GLCAT14A, GLCAT14B and GLCAT14C) in Arabidopsis thaliana, responsible for the addition of glucuronic acid to AG chains, were further investigated using single, double and triple glcat14 mutant plants. These proteins were localized to the Golgi apparatus when transiently expressed in Nicotiana benthamiana. Sugar analysis of AGP extracts from Arabidopsis stem, leaf and siliques showed a consistent reduction in glucuronic acid in glcat14 mutants relative to wild type, with concomitant effects resulting in tissue-specific alterations, especially in arabinose and galactose sugars. Although we observed defects in trichome branching in glca14a/b and glca14a/b/c mutants, scanning electron microscope analysis/energy dispersive microanalysis (SEM/EDX) showed no difference in the calcium content of trichomes in these mutants relative to wild type. Immunoblot analyses of the stem and leaf showed a reduction in AGPs as detected with the LM2 antibody in glcat14a/b and glcat14a/b/c mutants relative to wild type. The current work exemplifies the possibility of conducting structure-function assessment of cell wall biosynthetic genes to identify their physiological roles in plant growth and development.
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影响因子：
3.8
作者：
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通讯作者：
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影响因子：
56.9
作者：
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作者：
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影响因子：
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作者：
Ajayi OO;Showalter AM
通讯作者：
Showalter AM
DOI：
10.1105/tpc.20.00027
发表时间：
2020-10
期刊：
The Plant cell
影响因子：
--
作者：
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通讯作者：
Dupree P