Positional Stability and Membrane Occupancy Define Skin Fibroblast Homeostasis In Vivo.
Positional Stability and Membrane Occupancy Define Skin Fibroblast Homeostasis In Vivo.
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DOI：
10.1016/j.cell.2018.10.013
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发表时间：
2018-11-29
期刊：
影响因子：
64.5
通讯作者：
Greco V
中科院分区：
文献类型：
--
作者：
Marsh E;Gonzalez DG;Lathrop EA;Boucher J;Greco V
成纤维细胞是我们器官的基本细胞和结构组成部分。尽管取得了一些进展，但成纤维细胞用于维持体内稳态的关键行为仍然不清楚。在这里，通过跟踪活小鼠中相同的皮肤成纤维细胞，我们表明成纤维细胞的位置随着时间的推移是稳定的，尽管邻近的成纤维细胞的损失，这种稳定性仍然保持。相比之下，成纤维细胞膜在稳态期间是动态的，并且以Rac1依赖性方式延伸以填充丢失的相邻成纤维细胞的空间。尽管成纤维细胞核组织中的间隙逐渐积累，但在老化期间保持位置稳定性，同时继续保持膜占有率。这项工作将位置稳定性和细胞占有率定义为小鼠整个生命周期内体内皮肤成纤维细胞稳态的关键原则，并确定在没有迁移的情况下膜延伸为执行这些原则的核心细胞机制。皮肤成纤维细胞随着时间的推移保持其位置，但在损伤或老化期间，它们可以在空间上延伸以补偿邻近细胞的损失作为稳态机制。
Fibroblasts are an essential cellular and structural component of our organs. Despite several advances, the critical behaviors that fibroblasts utilize to maintain their homeostasis in vivo have remained unclear. Here, by tracking the same skin fibroblasts in live mice, we show that fibroblast position is stable over time and that this stability is maintained despite the loss of neighboring fibroblasts. In contrast, fibroblast membranes are dynamic during homeostasis and extend to fill the space of lost neighboring fibroblasts in a Rac1-dependent manner. Positional stability is sustained during aging despite a progressive accumulation of gaps in fibroblast nuclei organization, while membrane occupancy continues to be maintained. This work defines positional stability and cell occupancy as key principles of skin fibroblast homeostasis in vivo, throughout the lifespan of mice, and identifies membrane extension in the absence of migration as the core cellular mechanism to carry out these principles. Skin fibroblasts maintain their position over time, but upon injury or during aging, they can extend spatially to compensate for neighboring cell loss as a homeostatic mechanism.
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