A glycine-rich RNA-binding protein mediating cold-inducible suppression of mammalian cell growth.
A glycine-rich RNA-binding protein mediating cold-inducible suppression of mammalian cell growth.
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富含甘氨酸的RNA结合蛋白，介导了对哺乳动物细胞生长的冷诱导抑制。
DOI：
10.1083/jcb.137.4.899
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发表时间：
1997-05-19
影响因子：
7.8
通讯作者：
Fujita, J
中科院分区：
文献类型：
--
作者：
Nishiyama, H;Itoh, K;Kaneko, Y;Kishishita, M;Yoshida, O;Fujita, J
为了应对低环境温度，哺乳动物细胞以及微生物改变各种生理功能，但这些适应的分子机制才刚刚开始被理解。我们在这里报告的小鼠冷诱导RNA结合蛋白（cirp）cDNA的分离和调查其在哺乳动物细胞的冷应激反应中的作用。cirp cDNA编码一个18 kD的蛋白质，由一个氨基端的RNA结合结构域和一个羧基端的甘氨酸丰富的结构域组成，并表现出结构相似的一类胁迫诱导的RNA结合蛋白在植物中发现。免疫荧光显微镜显示CIRP定位于BALB/3 T3小鼠成纤维细胞的核浆中。当培养温度从37 ° C降低到32°C时，诱导了CIRP的表达，并且与37°C相比，BALB/3 T3细胞的生长受到损害。通过用反义寡脱氧核苷酸抑制CIRP的诱导，这种损害得到缓解，而CIRP的过表达导致在37°C下生长受损，细胞周期的G1期延长。这些结果表明CIRP在小鼠成纤维细胞的冷诱导的生长抑制中起重要作用。CIRP的鉴定可能为哺乳动物细胞冷反应的调控机制提供线索。
In response to low ambient temperature, mammalian cells as well as microorganisms change various physiological functions, but the molecular mechanisms underlying these adaptations are just beginning to be understood. We report here the isolation of a mouse cold-inducible RNA-binding protein (cirp) cDNA and investigation of its role in cold-stress response of mammalian cells. The cirp cDNA encoded an 18-kD protein consisting of an amino-terminal RNAbinding domain and a carboxyl-terminal glycine-rich domain and exhibited structural similarity to a class of stress-induced RNA-binding proteins found in plants. Immunofluorescence microscopy showed that CIRP was localized in the nucleoplasm of BALB/3T3 mouse fibroblasts. When the culture temperature was lowered from 37 to 32°C, expression of CIRP was induced and growth of BALB/3T3 cells was impaired as compared with that at 37°C. By suppressing the induction of CIRP with antisense oligodeoxynucleotides, this impairment was alleviated, while overexpression of CIRP resulted in impaired growth at 37°C with prolongation of G1 phase of the cell cycle. These results indicate that CIRP plays an essential role in cold-induced growth suppression of mouse fibroblasts. Identification of CIRP may provide a clue to the regulatory mechanisms of cold responses in mammalian cells.
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影响因子：
7.2
作者：
HEINTZEN, C;MELZER, S;STAIGER, D
通讯作者：
STAIGER, D
影响因子：
5.6
作者：
GLOFCHESKI, DJ;BORRELLI, MJ;KRUUV, J
通讯作者：
KRUUV, J
影响因子：
7.4
作者：
CARPENTER, CD;KREPS, JA;SIMON, AE
通讯作者：
SIMON, AE
影响因子：
3.5
作者：
DERRY, JMJ;KERNS, JA;FRANCKE, U
通讯作者：
FRANCKE, U
影响因子：
3.5
作者：
FUKAMIKOBAYASHI, K;TOMODA, S;GO, M
通讯作者：
GO, M