Virologic and serologic surveillance for human, swine and avian influenza virus infections among pigs in the north-central United States.
Virologic and serologic surveillance for human, swine and avian influenza virus infections among pigs in the north-central United States.
复制标题
DOI：
10.1007/s007050070098
复制
发表时间：
2000
影响因子：
2.7
通讯作者：
Karasin AI
中科院分区：
文献类型：
--
作者：
Olsen CW;Carey S;Hinshaw L;Karasin AI
猪的 流感病毒感染既是动物健康问题，也是公共卫生问题。因此，监测和确定猪流感病毒的特征对兽医界很重要，应该成为人类大流行防备计划的一部分。1976/1977和1988/1989年的研究表明，美国的猪通常感染经典的猪H1N1病毒，而人H3和禽流感病毒感染非常罕见。相比之下，在过去20年里，从欧洲和亚洲的猪身上频繁分离出人H3和禽流感H1病毒。从1997年9月到1998年8月，我们从美国中北部屠宰点的猪身上分离出26种流感病毒。26株毒株均为H1N1病毒，11株代表性毒株的血凝素基因和核蛋白基因的系统发育分析表明，这些毒株都是典型的猪H1病毒。然而，单抗分析显示，26种病毒的HA蛋白之间存在抗原性异质性。从血清学上看，2375头猪中有27.7%的猪具有抗猪瘟病毒血凝抑制抗体。然而，具有特别重要意义的是，对禽流感病毒H1(7.6%)和人H3病毒(8.0%)的血清阳性率显著高于以前的研究。
Influenza virus infection in pigs is both an animal health problem and a public health concern. As such, surveillance and characterization of influenza viruses in swine is important to the veterinary community and should be a part of human pandemic preparedness planning. Studies in 1976/1977 and 1988/1989 demonstrated that pigs in the U.S. were commonly infected with classical swine H1N1 viruses, whereas human H3 and avian influenza virus infections were very rare. In contrast, human H3 and avian H1 viruses have been isolated frequently from pigs in Europe and Asia over the last two decades. From September 1997 through August 1998, we isolated 26 influenza viruses from pigs in the north-central United States at the point of slaughter. All 26 isolates were H1N1 viruses, and phylogenetic analyses of the hemagglutinin and nucleoprotein genes from 11 representative viruses demonstrated that these were classical swine H1 viruses. However, monoclonal antibody analyses revealed antigenic heterogeneity among the HA proteins of the 26 viruses. Serologically, 27.7% of 2,375 pigs tested had hemagglutination-inhibiting antibodies against classical swine H1 influenza virus. Of particular significance, however, the rates of seropositivity to avian H1 (7.6%) and human H3 (8.0%) viruses were substantially higher than in previous studies.
登录
查看更多内容
影响因子：
3.8
作者：
Brown, IH;Ludwig, S;Alexander, DJ
通讯作者：
Alexander, DJ
影响因子：
3.7
作者：
BUCKLERWHITE, AJ;MURPHY, BR
通讯作者：
MURPHY, BR
影响因子：
1.5
作者：
DEA, S;BILODEAU, R;MARTINEAU, GP
通讯作者：
MARTINEAU, GP
DOI：
10.1073/pnas.80.22.6996
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BOTH, GW;SHI, CH;KILBOURNE, ED
通讯作者：
KILBOURNE, ED
影响因子：
64.5
作者：
CATON, AJ;BROWNLEE, GG;GERHARD, W
通讯作者：
GERHARD, W