Dissimilatory Sulfate Reduction Under High Pressure by Desulfovibrio alaskensis G20.
Dissimilatory Sulfate Reduction Under High Pressure by Desulfovibrio alaskensis G20.
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DOI：
10.3389/fmicb.2018.01465
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发表时间：
2018
影响因子：
5.2
通讯作者：
Coates JD
中科院分区：
文献类型：
--
作者：
Williamson AJ;Carlson HK;Kuehl JV;Huang LL;Iavarone AT;Deutschbauer A;Coates JD
生物酸化是油藏中硫酸盐还原微生物（SRMs）产生H2S的结果。H2S具有毒性、腐蚀性和爆炸性，因此对人员、生产设施和运输管道构成重大威胁。由于典型的油藏压力范围为10 ~ 50mpa，因此了解压力在SRM代谢中的作用对于改进防酸策略非常重要。为了探索压力的影响，我们在30°C的压力范围（0.1-14 MPa）下培养了油田SRM分离物Desulfovibrio alaskensis G20。观察到的微生物生长速率与压力成反比，并伴有硫酸盐和乳酸消耗速率的轻微降低。利用随机条形码转座子突变文库测序（RB-TnSeq）进行竞争适应度实验，发现了几个与鞭毛生物合成和组装相关的基因，这些基因在高压下很重要。利用单个转座子突变体证实了特定基因对适应度的影响。共聚焦显微镜显示，细胞聚集增强发生在压力下生长的后期。我们还评估了压力对SRM抑制剂效力的影响。剂量-反应实验表明，在14 MPa时，阿拉斯赤霉对抗生素氯霉素的敏感性降低了2倍。幸运的是，压力对常见的酸味控制剂硝酸盐或新兴的SRM抑制剂高氯酸盐、单氟磷酸盐或吡硫锌的抑制效力没有显著影响。我们的研究结果完善了高压环境下微生物硫酸盐还原的概念模型以及压力对酸阻剂效果的影响。
Biosouring results from production of H2S by sulfate-reducing microorganisms (SRMs) in oil reservoirs. H2S is toxic, corrosive, and explosive, and as such, represents a significant threat to personnel, production facilities, and transportation pipelines. Since typical oil reservoir pressures can range from 10 to 50 MPa, understanding the role that pressure plays in SRM metabolism is important to improving souring containment strategies. To explore the impact of pressure, we grew an oil-field SRM isolate, Desulfovibrio alaskensis G20, under a range of pressures (0.1–14 MPa) at 30°C. The observed microbial growth rate was an inverse function of pressure with an associated slight reduction in sulfate and lactate consumption rate. Competitive fitness experiments with randomly bar-coded transposon mutant library sequencing (RB-TnSeq) identified several genes associated with flagellar biosynthesis and assembly that were important at high pressure. The fitness impact of specific genes was confirmed using individual transposon mutants. Confocal microscopy revealed that enhanced cell aggregation occurs at later stages of growth under pressure. We also assessed the effect of pressure on SRM inhibitor potency. Dose-response experiments showed a twofold decrease in the sensitivity of D. alaskensis to the antibiotic chloramphenicol at 14 MPa. Fortuitously, pressure had no significant influence on the inhibitory potency of the common souring controlling agent nitrate, or the emerging SRM inhibitors perchlorate, monofluorophosphate, or zinc pyrithione. Our findings improve the conceptual model of microbial sulfate reduction in high-pressure environments and the influence of pressure on souring inhibitor efficacy.
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影响因子：
3.7
作者：
Martini S;Al Ali B;Garel M;Nerini D;Grossi V;Pacton M;Casalot L;Cuny P;Tamburini C
通讯作者：
Tamburini C
脱硫弧菌希尔顿伯勒的生物膜形成依赖于蛋白质丝
影响因子：
5.1
作者：
Clark, Melinda E.;Edelmann, Richard E.;Fields, Matthew W.
通讯作者：
Fields, Matthew W.
影响因子：
4.4
作者：
Hubert, Casey;Voordouw, Gerrit
通讯作者：
Voordouw, Gerrit
影响因子：
2.9
作者：
Martin, DD;Bartlett, DH;Roberts, MF
通讯作者：
Roberts, MF
影响因子：
4.4
作者：
He, Zhili;Zhou, Aifen;Zhou, Jizhong
通讯作者：
Zhou, Jizhong