Cardiovascular effect is independent of hemolytic toxicity of tentacle-only extract from the jellyfish Cyanea capillata.
Cardiovascular effect is independent of hemolytic toxicity of tentacle-only extract from the jellyfish Cyanea capillata.
复制标题
DOI：
10.1371/journal.pone.0043096
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Liming Z
中科院分区：
文献类型：
--
作者：
Liang X;Beilei W;Ying L;Qianqian W;Sihua L;Yang W;Guoyan L;Jia L;Xuting Y;Liming Z
我们以前的研究已经证实，从水母Cyanea capillata（Cyaneidae）的粗触手提取物（cTOE）同时表现出溶血和心血管毒性。因此，很难辨别cTOE引起心脏损伤的潜在活性成分。cTOE溶血毒性的灭活和大量沉淀的去除将有助于心血管成分的分离和心血管损伤机制的研究。在我们的研究中，经过一步碱变性和两次透析处理后，处理后的触手提取物（tTOE）的蛋白浓度约为cTOE的1/3，并且SDS-PAGE显示tTOE中的蛋白条带数量较少且密度较低。tTOE的溶血毒性完全丧失，而其心血管毒性保留良好。心功能、组织病理学和超微结构病理学的观察结果均支持tTOE具有显著的心血管毒性。tTOE对血气指标和电解质的影响远小于cTOE，但与正常值相比仍有显著性差异。综上所述，cTOE的心血管毒性可以独立于溶血毒性而存在，tTOE可以作为一种较好的毒液样品，用于水母心血管毒性蛋白的进一步纯化和机制研究。
Our previous studies have confirmed that the crude tentacle-only extract (cTOE) from the jellyfish Cyanea capillata (Cyaneidae) exhibits hemolytic and cardiovascular toxicities simultaneously. So, it is quite difficult to discern the underlying active component responsible for heart injury caused by cTOE. The inactivation of the hemolytic toxicity from cTOE accompanied with a removal of plenty of precipitates would facilitate the separation of cardiovascular component and the investigation of its cardiovascular injury mechanism. In our research, after the treatment of one-step alkaline denaturation followed by twice dialysis, the protein concentration of the treated tentacle-only extract (tTOE) was about 1/3 of cTOE, and SDS-PAGE showed smaller numbers and lower density of protein bands in tTOE. The hemolytic toxicity of tTOE was completely lost while its cardiovascular toxicity was well retained. The observations of cardiac function, histopathology and ultrastructural pathology all support tTOE with significant cardiovascular toxicity. Blood gas indexes and electrolytes changed far less by tTOE than those by cTOE, though still with significant difference from normal. In summary, the cardiovascular toxicity of cTOE can exist independently of the hemolytic toxicity and tTOE can be employed as a better venom sample for further purification and mechanism research on the jellyfish cardiovascular toxic proteins.
登录
查看更多内容
影响因子：
2.8
作者：
Edwards, LP;Whitter, E;Hessinger, DA
通讯作者：
Hessinger, DA
DOI：
10.1016/j.jchromb.2008.06.040
发表时间：
2008-08-01
影响因子：
3
作者：
Helmholz, Heike;Naatz, Stefanie;Prange, Andreas
通讯作者：
Prange, Andreas
影响因子：
2.8
作者：
Feng, Jinhua;Yu, Huahua;Li, Pengcheng
通讯作者：
Li, Pengcheng
影响因子：
2.8
作者：
Tibballs, James
通讯作者：
Tibballs, James
影响因子：
3.5
作者：
Kim, Euikyung;Lee, Seunghwan;Hodgson, Wayne C.
通讯作者：
Hodgson, Wayne C.